PUBLICATION
Mitochondria-targeted fluorescent probe for simultaneously imaging viscosity and sulfite in inflammation models
- Authors
- Peng, Z., Zhang, D., Yang, H., Zhou, Z., Wang, F., Wang, Z., Ren, J., Wang, E.
- ID
- ZDB-PUB-240507-6
- Date
- 2024
- Source
- The Analyst 149(12): 3356-3362 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- RAW 264.7 Cells
- Mitochondria*/chemistry
- Mitochondria*/metabolism
- Sulfur Dioxide/analysis
- Sulfur Dioxide/chemistry
- Fluorescent Dyes*/chemical synthesis
- Fluorescent Dyes*/chemistry
- Fluorescent Dyes*/toxicity
- Optical Imaging/methods
- Sulfites*/analysis
- Sulfites*/chemistry
- Mice
- Animals
- Inflammation*/chemically induced
- Viscosity
- Humans
- Lipopolysaccharides
- Zebrafish*
- PubMed
- 38712511 Full text @ Analyst
Citation
Peng, Z., Zhang, D., Yang, H., Zhou, Z., Wang, F., Wang, Z., Ren, J., Wang, E. (2024) Mitochondria-targeted fluorescent probe for simultaneously imaging viscosity and sulfite in inflammation models. The Analyst. 149(12):3356-3362.
Abstract
Many diseases in the human body are related to the overexpression of viscosity and sulfur dioxide. Therefore, it is essential to develop rapid and sensitive fluorescent probes to detect viscosity and sulfur dioxide. In the present work, we developed a dual-response fluorescent probe (ES) for efficient detection of viscosity and sulfur dioxide while targeting mitochondria well. The probe generates intramolecular charge transfer by pushing and pulling the electron-electron system, and the ICT effect is destroyed and the fluorescence quenched upon reaction with sulfite. The rotation of the molecule is inhibited in the high-viscosity system, producing a bright red light. In addition, the probe has good biocompatibility and can be used to detect sulfite in cells, zebrafish and mice, as well as upregulation of viscosity in LPS-induced inflammation models. We expect that the dual response fluorescent probe ES will be able to detect viscosity and sulfite efficiently, providing an effective means of detecting viscosity and sulfite-related diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping