PUBLICATION
Notochord segmentation in zebrafish controlled by iterative mechanical signaling
- Authors
- Wopat, S., Adhyapok, P., Daga, B., Crawford, J.M., Norman, J., Bagwell, J., Peskin, B., Magre, I., Fogerson, S.M., Levic, D.S., Di Talia, S., Kiehart, D.P., Charbonneau, P., Bagnat, M.
- ID
- ZDB-PUB-240503-11
- Date
- 2024
- Source
- Developmental Cell 59(14): 1860-1875.e5 (Journal)
- Registered Authors
- Bagnat, Michel
- Keywords
- extracellular matrix, morphogenesis, myosepta, notochord, segmentation, somites, tissue mechanics, zebrafish
- MeSH Terms
-
- Animals
- Body Patterning*
- Embryo, Nonmammalian/metabolism
- Extracellular Matrix/metabolism
- Gene Expression Regulation, Developmental
- Notochord*/embryology
- Notochord*/metabolism
- Signal Transduction
- Somites*/embryology
- Somites*/metabolism
- Spine*/embryology
- Zebrafish*/embryology
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 38697108 Full text @ Dev. Cell
Citation
Wopat, S., Adhyapok, P., Daga, B., Crawford, J.M., Norman, J., Bagwell, J., Peskin, B., Magre, I., Fogerson, S.M., Levic, D.S., Di Talia, S., Kiehart, D.P., Charbonneau, P., Bagnat, M. (2024) Notochord segmentation in zebrafish controlled by iterative mechanical signaling. Developmental Cell. 59(14):1860-1875.e5.
Abstract
In bony fishes, patterning of the vertebral column, or spine, is guided by a metameric blueprint established in the notochord sheath. Notochord segmentation begins days after somitogenesis concludes and can occur in its absence. However, somite patterning defects lead to imprecise notochord segmentation, suggesting that these processes are linked. Here, we identify that interactions between the notochord and the axial musculature ensure precise spatiotemporal segmentation of the zebrafish spine. We demonstrate that myoseptum-notochord linkages drive notochord segment initiation by locally deforming the notochord extracellular matrix and recruiting focal adhesion machinery at these contact points. Irregular somite patterning alters this mechanical signaling, causing non-sequential and dysmorphic notochord segmentation, leading to altered spine development. Using a model that captures myoseptum-notochord interactions, we find that a fixed spatial interval is critical for driving sequential segment initiation. Thus, mechanical coupling of axial tissues facilitates spatiotemporal spine patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping