PUBLICATION
Antigen presentation plays positive roles in the regenerative response to cardiac injury in zebrafish
- Authors
- Cardeira-da-Silva, J., Wang, Q., Sagvekar, P., Mintcheva, J., Latting, S., Günther, S., Ramadass, R., Yekelchyk, M., Preussner, J., Looso, M., Junker, J.P., Stainier, D.Y.R.
- ID
- ZDB-PUB-240430-7
- Date
- 2024
- Source
- Nature communications 15: 36373637 (Journal)
- Registered Authors
- Keywords
- none
- Datasets
- GEO:GSE232061, GEO:GSE232059, GEO:GSE230669, GEO:GSE232060
- MeSH Terms
-
- Heart/physiology
- Heart/physiopathology
- Cell Proliferation
- Antigen Presentation*/immunology
- Myocytes, Cardiac/immunology
- Myocytes, Cardiac/metabolism
- Adaptive Immunity
- Heart Injuries*/immunology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish*
- Antigens, Differentiation, B-Lymphocyte/genetics
- Antigens, Differentiation, B-Lymphocyte/metabolism
- Immunity, Innate
- Regeneration*/immunology
- CD4-Positive T-Lymphocytes/immunology
- Mice
- Animals
- Animals, Genetically Modified
- Histocompatibility Antigens Class II*/genetics
- Histocompatibility Antigens Class II*/immunology
- Histocompatibility Antigens Class II*/metabolism
- Mutation
- PubMed
- 38684665 Full text @ Nat. Commun.
Citation
Cardeira-da-Silva, J., Wang, Q., Sagvekar, P., Mintcheva, J., Latting, S., Günther, S., Ramadass, R., Yekelchyk, M., Preussner, J., Looso, M., Junker, J.P., Stainier, D.Y.R. (2024) Antigen presentation plays positive roles in the regenerative response to cardiac injury in zebrafish. Nature communications. 15:36373637.
Abstract
In contrast to adult mammals, adult zebrafish can fully regenerate injured cardiac tissue, and this regeneration process requires an adequate and tightly controlled immune response. However, which components of the immune response are required during regeneration is unclear. Here, we report positive roles for the antigen presentation-adaptive immunity axis during zebrafish cardiac regeneration. We find that following the initial innate immune response, activated endocardial cells (EdCs), as well as immune cells, start expressing antigen presentation genes. We also observe that T helper cells, a.k.a. Cd4+ T cells, lie in close physical proximity to these antigen-presenting EdCs. We targeted Major Histocompatibility Complex (MHC) class II antigen presentation by generating cd74a; cd74b mutants, which display a defective immune response. In these mutants, Cd4+ T cells and activated EdCs fail to efficiently populate the injured tissue and EdC proliferation is significantly decreased. cd74a; cd74b mutants exhibit additional defects in cardiac regeneration including reduced cardiomyocyte dedifferentiation and proliferation. Notably, Cd74 also becomes activated in neonatal mouse EdCs following cardiac injury. Altogether, these findings point to positive roles for antigen presentation during cardiac regeneration, potentially involving interactions between activated EdCs, classical antigen-presenting cells, and Cd4+ T cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping