PUBLICATION
An Undergraduate Course in CRISPR/Cas9-Mediated Gene Editing in Zebrafish
- Authors
- Srivastava, R., Davison, C.W., Krull, A.G., Entriken, S.M., Zumbrock, A., Cortes Hidalgo, M.D., Adair, K.J., Escherich, A.M., Lara, J.N., Neverman, E.C., Hodnefield, M., McElligtot, E., Sandquist, E.J., Ogilvie, C., Lafontant, P., Essner, J.J.
- ID
- ZDB-PUB-240416-21
- Date
- 2024
- Source
- Zebrafish 21: 162170162-170 (Journal)
- Registered Authors
- Essner, Jeffrey
- Keywords
- CRISPR, Cas9, gene editing, undergraduate course, zebrafish
- MeSH Terms
-
- Gene Editing*/methods
- Humans
- CRISPR-Cas Systems*
- Students
- Animals
- Zebrafish/genetics
- RNA, Guide, CRISPR-Cas Systems
- PubMed
- 38621214 Full text @ Zebrafish
Citation
Srivastava, R., Davison, C.W., Krull, A.G., Entriken, S.M., Zumbrock, A., Cortes Hidalgo, M.D., Adair, K.J., Escherich, A.M., Lara, J.N., Neverman, E.C., Hodnefield, M., McElligtot, E., Sandquist, E.J., Ogilvie, C., Lafontant, P., Essner, J.J. (2024) An Undergraduate Course in CRISPR/Cas9-Mediated Gene Editing in Zebrafish. Zebrafish. 21:162170162-170.
Abstract
We have developed a one-credit semester-long research experience for undergraduate students that involves the use of CRISPR/Cas9 to edit genes in zebrafish. The course is available to students at all stages of their undergraduate training and can be taken up to four times. Students select a gene of interest to edit as the basis of their semester-long project. To select a gene, exploration of developmental processes and human disease is encouraged. As part of the course, students use basic bioinformatic tools, design guide RNAs, inject zebrafish embryos, and analyze both the molecular consequences of gene editing and phenotypic outcomes. Over the 10 years we have offered the course, enrollment has grown from less than 10 students to more than 60 students per semester. Each year, we choose a different gene editing strategy to explore based on recent publications of gene editing methodologies. These have included making CRISPants, targeted integrations, and large gene deletions. In this study, we present how we structure the course and our assessment of the course over the past 3 years.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping