PUBLICATION
The serotonergic neurons derived from rhombomere 2 are localized in the median raphe and project to the dorsal pallium in zebrafish
- Authors
- Shibayama, K., Nakajo, H., Tanimoto, Y., Kakinuma, H., Shiraki, T., Tsuboi, T., Okamoto, H.
- ID
- ZDB-PUB-240307-13
- Date
- 2024
- Source
- Neuroscience research 205: 27-33 (Journal)
- Registered Authors
- Kakinuma, Hisaya, Okamoto, Hitoshi, Shibayama, Kotaro, Shiraki, Toshiyuki, Tanimoto, Yuki
- Keywords
- Dorsal pallium, Median raphe, Neural circuit, Rhombomere, Serotonin, Zebrafish
- MeSH Terms
-
- Habenula/cytology
- Habenula/physiology
- Serotonin/metabolism
- Serotonergic Neurons*/physiology
- Zebrafish*
- Animals, Genetically Modified*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Animals
- Raphe Nuclei*/cytology
- Neural Pathways/physiology
- PubMed
- 38447890 Full text @ Neurosci. Res.
Citation
Shibayama, K., Nakajo, H., Tanimoto, Y., Kakinuma, H., Shiraki, T., Tsuboi, T., Okamoto, H. (2024) The serotonergic neurons derived from rhombomere 2 are localized in the median raphe and project to the dorsal pallium in zebrafish. Neuroscience research. 205:27-33.
Abstract
The serotonergic neurons in the raphe nucleus are implicated in various cognitive functions such as learning and emotion. In vertebrates, the raphe nucleus is divided into the dorsal raphe and the median raphe. In contrast to the abundance of knowledge on the functions of the dorsal raphe, the roles of the serotonergic neurons in the median raphe are relatively unknown. The studies using zebrafish revealed that the median raphe serotonergic neurons receive input from the two distinct pathways from the habenula and the IPN. The use of zebrafish may reveal the function of the Hb-IPN-median raphe pathway. To clarify the functions of the median raphe serotonergic neurons, it is necessary to distinguish them from those in the dorsal raphe. Most median raphe serotonergic neurons originate from rhombomere 2 in mice, and we generated the transgenic zebrafish which can label the serotonergic neurons derived from rhombomere 2. In this study, we found the serotonergic neurons derived from rhombomere 2 are localized in the median raphe and project axons to the rostral dorsal pallium in zebrafish. This study suggests that this transgenic system has the potential to specifically reveal the function and information processing of the Hb-IPN-raphe-telencephalon circuit in learning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping