PUBLICATION
Behavioral and neurotransmitter changes on antiepileptic drugs treatment in the zebrafish pentylenetetrazol-induced seizure model
- Authors
- Okanari, K., Teranishi, H., Umeda, R., Shikano, K., Inoue, M., Hanada, T., Ihara, K., Hanada, R.
- ID
- ZDB-PUB-240226-5
- Date
- 2024
- Source
- Behavioural brain research 464: 114920 (Journal)
- Registered Authors
- Hanada, Reiko, Hanada, Toshikatsu
- Keywords
- Antiseizure drugs, Epilepsy, Neurotransmitter, Pentylenetetrazol, Seizure, Zebrafish
- MeSH Terms
-
- Animals
- Anticonvulsants*/adverse effects
- Carbamazepine/pharmacology
- Epilepsy*
- Glutamic Acid
- Levetiracetam/pharmacology
- Levetiracetam/therapeutic use
- Neurotransmitter Agents
- Pentylenetetrazole/toxicity
- Seizures/chemically induced
- Seizures/drug therapy
- Serotonin
- Zebrafish
- gamma-Aminobutyric Acid
- PubMed
- 38403178 Full text @ Behav. Brain Res.
Citation
Okanari, K., Teranishi, H., Umeda, R., Shikano, K., Inoue, M., Hanada, T., Ihara, K., Hanada, R. (2024) Behavioral and neurotransmitter changes on antiepileptic drugs treatment in the zebrafish pentylenetetrazol-induced seizure model. Behavioural brain research. 464:114920.
Abstract
Epilepsy, a recurrent neurological disorder involving abnormal neurotransmitter kinetics in the brain, has emerged as a global health concern. The mechanism of epileptic seizures is thought to involve a relative imbalance between excitatory and inhibitory neurotransmitters. Despite the recent advances in clinical and basic research on the pathogenesis of epilepsy, the complex relationship between the neurotransmitter changes and behavior with and without antiepileptic drugs (AEDs) during seizures remains unclear. To investigate the effects of AEDs such as levetiracetam (LEV), carbamazepine (CBZ), and fenfluramine (FFR) on key neurotransmitters in the pentylenetetrazol (PTZ)-induced seizures in adult zebrafish, we examined the changes in glutamic acid, gamma-aminobutyric acid (GABA), serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), choline, acetylcholine, norepinephrine, dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), and adenosine. In this study, we observed that 5-HT and DA levels in the brain increased immediately after PTZ-induced seizures. Behavioral tests clearly showed that all of these AEDs suppressed the PTZ-induced seizures. Upon treatment of PTZ-induced seizures with these AEDs, CBZ decreased the glutamic acid and FFR increased the GABA levels; however, no neurotransmitter changes were observed in the brain after LEV administration. Thus, we demonstrated a series of neurotransmitter changes linked to behavioral changes during PTZ-induced epileptic seizures when LEV, CBZ, or FFR were administered. These findings will lead to a more detailed understanding of the pathogenesis of epilepsy associated with behavioral and neurotransmitter changes under AED treatment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping