PUBLICATION
Hypolipidemic and Antithrombotic Effect of 6'-O-Caffeoylarbutin from Vaccinium dunalianum Based on Zebrafish Model, Network Pharmacology, and Molecular Docking
- Authors
- Wu, B., Li, C., Kan, H., Zhang, Y., Rao, X., Liu, Y., Zhao, P.
- ID
- ZDB-PUB-240225-21
- Date
- 2024
- Source
- Molecules 29(4): (Journal)
- Registered Authors
- Keywords
- 6′-O-Caffeoylarbutin, Vaccinium dunalianum Wight, antithrombotic, hypolipidemic, molecular docking, network pharmacology
- MeSH Terms
-
- Fibrinolytic Agents/pharmacology
- Network Pharmacology
- Zebrafish
- Molecular Docking Simulation
- Drugs, Chinese Herbal*
- Arbutin*/analogs & derivatives
- Thrombosis*/drug therapy
- Hyperlipidemias*/drug therapy
- Animals
- Caffeic Acids*
- PubMed
- 38398534 Full text @ Molecules
Citation
Wu, B., Li, C., Kan, H., Zhang, Y., Rao, X., Liu, Y., Zhao, P. (2024) Hypolipidemic and Antithrombotic Effect of 6'-O-Caffeoylarbutin from Vaccinium dunalianum Based on Zebrafish Model, Network Pharmacology, and Molecular Docking. Molecules. 29(4):.
Abstract
Vaccinium dunalianum leaf buds make one of the most commonly used herbal teas of the Yi people in China, which is used to treat articular rheumatism, relax tendons, and stimulates blood circulation in the body. In addition, 6'-O-caffeoylarbutin (CA) is a standardized extract of V. dunalianum, which has been found in dried leaf buds, reaching levels of up to 31.76%. Because of the uncommon phenomenon, it is suggested that CA may have a potential therapeutic role in hyperlipidemia and thrombosis. This study was designed to study the efficacy of CA on treating hyperlipidemia and thrombosis and the possible mechanisms behind these effects. Hyperlipidemia and thrombosis zebrafish models were treated with CA to observe variations of the integrated optical density within the vessels and the intensity of erythrocyte staining within the hearts. The possible mechanisms were explored using network pharmacology and molecular docking. The results demonstrate that CA exhibits an excellent hypolipidemic effect on zebrafish at concentrations ranging from 3.0 to 30.0 μg/mL and shows thrombosis inhibitory activity in zebrafish at a concentration of 30.0 μg/mL, with an inhibition rate of 44%. Moreover, network pharmacological research shows that MMP9, RELA, MMP2, PRKCA, HSP90AA1, and APP are major targets of CA for therapy of hyperlipidemia and thrombosis, and may relate to pathways in cancer, chemical carcinogenesis-receptor activation, estrogen signaling pathway, and the AGE-RAGE signaling pathway in diabetic complications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping