PUBLICATION
Furan-based Chalcone Annihilates the Multi-Drug-Resistant Pseudomonas aeruginosa and Protects Zebra Fish Against its Infection
- Authors
- Nayak, S.P.R.R., Basty, C., Boopathi, S., Dhivya, L.S., Alarjani, K.M., Gawwad, M.R.A., Hager, R., Kathiravan, M.K., Arockiaraj, J.
- ID
- ZDB-PUB-240222-26
- Date
- 2024
- Source
- Journal of microbiology (Seoul, Korea) 62(2): 75-89 (Journal)
- Registered Authors
- Keywords
- Antimicrobial agent, Chalcone, Multidrug resistant bacteria, Oxidative stress, Pseudomonas aeruginosa
- MeSH Terms
-
- Adult
- Animals
- Anti-Bacterial Agents/metabolism
- Anti-Bacterial Agents/pharmacology
- Bacteria
- Chalcone*/metabolism
- Chalcone*/pharmacology
- Chalcones*/metabolism
- Chalcones*/pharmacology
- Humans
- Microbial Sensitivity Tests
- Pseudomonas aeruginosa/metabolism
- Zebrafish
- PubMed
- 38383881 Full text @ J Microbiol
Citation
Nayak, S.P.R.R., Basty, C., Boopathi, S., Dhivya, L.S., Alarjani, K.M., Gawwad, M.R.A., Hager, R., Kathiravan, M.K., Arockiaraj, J. (2024) Furan-based Chalcone Annihilates the Multi-Drug-Resistant Pseudomonas aeruginosa and Protects Zebra Fish Against its Infection. Journal of microbiology (Seoul, Korea). 62(2):75-89.
Abstract
The emergence of carbapenem-resistant Pseudomonas aeruginosa, a multi-drug-resistant bacteria, is becoming a serious public health concern. This bacterium infects immunocompromised patients and has a high fatality rate. Both naturally and synthetically produced chalcones are known to have a wide array of biological activities. The antibacterial properties of synthetically produced chalcone were studied against P. aeruginosa. In vitro, study of the compound (chalcone derivative named DKO1), also known as (2E)-1-(5-methylfuran-2-yl)-3-(4-nitrophenyl) prop-2-en-1-one, had substantial antibacterial and biofilm disruptive action. DKO1 effectively shielded against P. aeruginosa-induced inflammation, oxidative stress, lipid peroxidation, and apoptosis in zebrafish larvae. In adult zebrafish, the treatment enhanced the chances of survivability and reduced the sickness-like behaviors. Gene expression, biochemical analysis, and histopathology studies found that proinflammatory cytokines (TNF-α, IL-1β, IL-6, iNOS) were down regulated; antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) levels increased, and histoarchitecture was restored in zebrafish. The data indicate that DKO1 is an effective antibacterial agent against P. aeruginosa demonstrated both in vitro and in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping