PUBLICATION
Caspase-8 dependent apoptosis contributes to dyskinesia caused by muscle defects and neurotoxicity in zebrafish exposed to zearalenone
- Authors
- Wang, Y., Li, S., Cheng, Z., Zhang, Z., Xu, Y., Zhang, H., Xu, T., Chen, J., Yin, D., Yan, W., Huang, H.
- ID
- ZDB-PUB-240222-24
- Date
- 2024
- Source
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 186: 114516 (Journal)
- Registered Authors
- Huang, Huizhe
- Keywords
- Apoptosis, Dyskinesia, Muscle toxicity, Neurotoxicity, Zearalenone
- MeSH Terms
-
- Animals
- Apoptosis
- Caspase 8/genetics
- Caspase 8/metabolism
- Dyskinesias*
- Humans
- Larva
- Muscles/metabolism
- Mycotoxins/chemistry
- Mycotoxins/metabolism
- Zearalenone*/metabolism
- Zearalenone*/toxicity
- Zebrafish
- PubMed
- 38382872 Full text @ Food Chem. Toxicol.
Citation
Wang, Y., Li, S., Cheng, Z., Zhang, Z., Xu, Y., Zhang, H., Xu, T., Chen, J., Yin, D., Yan, W., Huang, H. (2024) Caspase-8 dependent apoptosis contributes to dyskinesia caused by muscle defects and neurotoxicity in zebrafish exposed to zearalenone. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 186:114516.
Abstract
Zearalenone (ZEA), one of the usual mycotoxins, has been recognized in many areas and crops, posing a significant threat to the living organisms even to human beings. However, the mechanisms of locomotive defects remain unknown. Herein, zebrafish larvae was employed to investigate ZEA effects on developmental indexes, muscle and neural toxicity, apoptosis, transcriptome and motor behaviors of zebrafish larvae. Zebrafish larvae exposed to ZEA (0, 0.5, 1, 2 and 4 μM) showed no change in survival rate, but the malformation rate of zebrafish larvae increased dramatically manifesting with severe body bending and accomplished with adverse effects on hatching rate and body length. Moreover, the larvae manifested with defective muscle and abnormal neural development, resulting in decreased swimming ability, which probably due to the abnormal overactivation of apoptosis. And this was confirmed by enriched caspase 8-mediated apoptosis signaling pathway in the following transcriptome analysis. Meanwhile, there was a recovery in swimming behaviors in the larvae co-exposed in ZEA and caspase 8 inhibitor. These findings provide an important evidence for risk assessment and potential treatment target of ZEA exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping