PUBLICATION

Whole-Transcriptome Analysis Reveals the RNA Profiles in Mouse Bone Marrow Mesenchymal Stem Cells or Zebrafish Embryos After Exposure to Environmental Level of Tributyltin

Authors
Li, P., Chen, C.Z., Liu, L., Li, Z.H.
ID
ZDB-PUB-240212-6
Date
2024
Source
Bulletin of environmental contamination and toxicology   112: 3434 (Journal)
Registered Authors
Keywords
Mouse BMSCs, Non-coding RNA, Tributyltin, Whole Transcriptome, Zebrafish Embryos
MeSH Terms
  • Zebrafish/metabolism
  • Animals
  • MicroRNAs*/genetics
  • MicroRNAs*/metabolism
  • RNA, Messenger/metabolism
  • Trialkyltin Compounds*
  • Mice
  • Gene Expression Profiling
  • RNA, Circular/genetics
  • RNA, Circular/metabolism
  • RNA, Long Noncoding*/genetics
  • RNA, Long Noncoding*/metabolism
  • Mesenchymal Stem Cells*/metabolism
PubMed
38342962 Full text @ Bull. Environ. Contam. Toxicol.
Abstract
To understand the underlying molecular mechanisms, mouse bone marrow mesenchymal stem cells (BMSCs) and zebrafish embryos were exposed to the control group and Tributyltin (TBT) group (10 ng/L, environmental concentration) for 48 h, respectively. The expression profiles of RNAs were investigated using whole-transcriptome analysis in mouse BMSCs or zebrafish embryos after TBT exposure. For mouse BMSCs, the results showed 2,449 differentially expressed (DE) mRNAs, 59 DE miRNAs, 317 DE lncRNAs, and 15 circRNAs. Similarly, for zebrafish embryos, the results showed 1,511 DE mRNAs, 4 DE miRNAs, 272 DE lncRNAs, and 28 circRNAs. According to KEGG pathway analysis showed that DE RNAs were mainly associated with immune responses, signaling, and cellular interactions. Competing endogenous RNA (ceRNA) network analysis revealed that the regulatory network of miRNA-circRNA constructed in zebrafish embryos was more complex compared to that of mouse BMSCs.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping