PUBLICATION
Nontargeted metabolomic insights into the behavioral effects of 5-MeO-MiPT in zebrafish (Danio rerio)
- Authors
- Zhao, S., Chen, J., Zhong, C., Meng, L., Wang, Y.
- ID
- ZDB-PUB-240201-3
- Date
- 2024
- Source
- Ecotoxicology and environmental safety 272: 116044116044 (Journal)
- Registered Authors
- Keywords
- Adult zebrafish；behavioral changes；UPLC-Q-Orbitrap-HRMS, Untargeted metabolomics；Brain-Liver Pathway Injury
- MeSH Terms
-
- 5-Methoxytryptamine/analogs & derivatives*
- Animals
- Liver/metabolism
- Metabolomics/methods
- Tryptamines*/metabolism
- Tryptamines*/toxicity
- Zebrafish*/metabolism
- PubMed
- 38295732 Full text @ Ecotoxicol. Environ. Saf.
Citation
Zhao, S., Chen, J., Zhong, C., Meng, L., Wang, Y. (2024) Nontargeted metabolomic insights into the behavioral effects of 5-MeO-MiPT in zebrafish (Danio rerio). Ecotoxicology and environmental safety. 272:116044116044.
Abstract
5-Methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) is a novel psychoactive substance exhibiting a tryptamine structure. Despite its increasing prevalence, the environmental impact of 5-MeO-MiPT remains unexplored. Our prior investigation revealed that 5-MeO-MiPT induced inhibited spontaneous movement and prompted anxiety-like behavior in adult zebrafish-a validated toxicological model. To elucidate this phenomenon and establish a correlation between metabolomics and behavioral changes induced by 5-MeO-MiPT, zebrafish were administered varying drug concentrations. Zebrafishes were subjected to injections of different 5-MeO-MiPT concentrations. Subsequent metabolomic analysis of endogenous metabolites affected by the drug unveiled substantial variations in metabolic levels between the control group and the drug-injected cohorts. A total of 22 distinct metabolites emerged as potential biomarkers. Further scrutiny identified seven pathways significantly influenced by 5-MeO-MiPT. A focused exploration into amino acid metabolism, lipid metabolism, and energy metabolism unveiled that the metabolic repercussions of 5-MeO-MiPT on zebrafish resulted in observable brain damage. Notably, the study identified a consequential disruption in the liver-brain pathway. The comprehensive metabolomic approach employed herein effectively discerned the impact of 5-MeO-MiPT on zebrafish metabolism. This approach also shed light on the mechanism underpinning the anxiety-like behavior observed in zebrafish post-drug injection. Specifically, our findings indicate that 5-MeO-MiPT induces brain damage, particularly within the liver-brain pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping