PUBLICATION
Zebrafish prss59.1 is involved in chorion development
- Authors
- Rezanujjaman, M., Pachoensuk, T., Forhad Hossain, M., Maisum Sarwar Jyoti, M., Rubel Rana, M., Tsutsumi, E., Mouri, T., Bramastri Susilo, M., Wanlada, K., Yamamoto, C., Hasan Ali, M., Tokumoto, T.
- ID
- ZDB-PUB-240129-8
- Date
- 2024
- Source
- General and comparative endocrinology 349: 114453 (Journal)
- Registered Authors
- Pachoensuk, Theeranukul, Tokumoto, Toshinobu
- Keywords
- Chorion, Fertilization, Prss59.1, Zebrafish
- MeSH Terms
-
- Animals
- Chorion/chemistry
- Chorion/physiology
- Female
- Fertilization*
- Homozygote
- Zebrafish*/physiology
- PubMed
- 38281702 Full text @ Gen. Comp. Endocrinol.
Citation
Rezanujjaman, M., Pachoensuk, T., Forhad Hossain, M., Maisum Sarwar Jyoti, M., Rubel Rana, M., Tsutsumi, E., Mouri, T., Bramastri Susilo, M., Wanlada, K., Yamamoto, C., Hasan Ali, M., Tokumoto, T. (2024) Zebrafish prss59.1 is involved in chorion development. General and comparative endocrinology. 349:114453.
Abstract
The prss59.1 gene was identified as one of 11 genes that were highly upregulated during the induction of ovulation in zebrafish by using an in vivo ovulation assay. Previously, we conducted biochemical characterization of Prss59.1 and revealed it to be a trypsin-like proteolytic enzyme. In this study, we established a prss59.1 gene knockout strain using the CRISPR/Cas9 system. Phenotypic analysis of prss59.1 knockout fish showed that prss59.1 is associated with chorion elevation, a prominent event in egg activation during fertilization. The chorions of heterozygous and homozygous prss59.1 mutant zebrafish were smaller than those of the wild type. The results suggested that Prss59.1 is necessary for chorion expansion. The homozygous prss59.1 mutant strain, with a small chorion, showed an extremely low survival rate. Fiber-supported knob-like structures (KS) on the chorion showed an abnormal structure in prss59.1 mutants. Prss59.1 was detected in the KS on the chorion. The pores on the chorion were smaller in the prss59.1 mutants than in the wild type. Transmission electron microscopy (TEM) observations of the cross sections of the chorions showed abnormalities in the chorion structure in prss59.1 mutants. These results demonstrated that Prss59.1 is involved in chorion elevation and in proper formation of the chorion, which is necessary for embryo development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping