PUBLICATION
Xanthohumol, a prenylated flavonoid from hops (Humulus lupulus L.) exerts multidirectional pro-healing properties towards damaged zebrafish hair cells by regulating the innate immune response
- Authors
- Kasica, N., Kaleczyc, J.
- ID
- ZDB-PUB-240112-11
- Date
- 2024
- Source
- Toxicology and applied pharmacology 483: 116809 (Journal)
- Registered Authors
- Kasica, Natalia
- Keywords
- Apoptosis, Innate immune system, Interleukins, Macrophages, Neutrophiles, Regeneration, Stat Family, Xanthohumol, Zebrafish
- MeSH Terms
-
- Animals
- Flavonoids/chemistry
- Hair/metabolism
- Humulus*/chemistry
- Humulus*/metabolism
- Immunity, Innate
- Inflammation/chemically induced
- Propiophenones*/chemistry
- Propiophenones*/metabolism
- Propiophenones*/toxicity
- Zebrafish/metabolism
- PubMed
- 38211931 Full text @ Tox. App. Pharmacol.
Citation
Kasica, N., Kaleczyc, J. (2024) Xanthohumol, a prenylated flavonoid from hops (Humulus lupulus L.) exerts multidirectional pro-healing properties towards damaged zebrafish hair cells by regulating the innate immune response. Toxicology and applied pharmacology. 483:116809.
Abstract
Xanthohumol (XN) is a prominent prenylated flavonoid present in the hop plant (Humulus lupulus L.). Despite undoubted pro-healing properties of hop plant, there is still a need for clinical investigations confirming these effects as well as the underlying molecular mechanisms. The present study was designed to (1) establish the role of XN in non-invasive inflammation induced by chemical damage to zebrafish hair cells, (2) clarify if it influences cell injury severity, neutrophil migration, macrophage activation, cell regeneration, and (3) find out whether it modulates the gene expression profile of chosen immune and stress response markers. All experiments were performed on 3 dpf zebrafish larvae. After fertilization the embryos were transferred to appropriate XN solutions (0.1 μM, 0.3 μM and 0.5 μM). The 40 min 10 μM CuSO4 exposure evoked severe damage to posterior lateral line hair cells triggering a robust acute inflammatory response. Four readouts were selected as the indicators of XN role in the process of inflammation: 1) hair cell death, 2) neutrophil migration towards damaged hair cells, 3) macrophage activation and recruitment to damaged hair cells, 4) hair cell regeneration. The assessments involved in vivo confocal microscopy imaging and qPCR based molecular analysis. It was demonstrated that XN (1) influences death pathway of damaged hair cells by redirecting their severe necrotic phenotype into apoptotic one, (2) impacts the immune response via regulating neutrophil migration, macrophage recruitment and activation (3) modulates gene expression of immune system markers and (4) accelerates hair cell regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping