PUBLICATION
Nlrc3 signaling is indispensable for hematopoietic stem cell emergence via Notch signaling in vertebrates
- Authors
- Cai, S., Li, H., Tie, R., Shan, W., Luo, Q., Wang, S., Feng, C., Chen, H., Zhang, M., Xu, Y., Li, X., Chen, M., Lu, J., Qian, P., Huang, H.
- ID
- ZDB-PUB-240105-15
- Date
- 2024
- Source
- Nature communications 15: 226226 (Journal)
- Registered Authors
- Keywords
- none
- Datasets
- GEO:GSE248871
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics
- Hematopoiesis/genetics
- Hematopoietic Stem Cells*/metabolism
- Mice
- Receptors, Notch/metabolism
- Signal Transduction
- Zebrafish*
- PubMed
- 38172511 Full text @ Nat. Commun.
Citation
Cai, S., Li, H., Tie, R., Shan, W., Luo, Q., Wang, S., Feng, C., Chen, H., Zhang, M., Xu, Y., Li, X., Chen, M., Lu, J., Qian, P., Huang, H. (2024) Nlrc3 signaling is indispensable for hematopoietic stem cell emergence via Notch signaling in vertebrates. Nature communications. 15:226226.
Abstract
Hematopoietic stem and progenitor cells generate all the lineages of blood cells throughout the lifespan of vertebrates. The emergence of hematopoietic stem and progenitor cells is finely tuned by a variety of signaling pathways. Previous studies have revealed the roles of pattern-recognition receptors such as Toll-like receptors and RIG-I-like receptors in hematopoiesis. In this study, we find that Nlrc3, a nucleotide-binding domain leucine-rich repeat containing family gene, is highly expressed in hematopoietic differentiation stages in vivo and vitro and is required in hematopoiesis in zebrafish. Mechanistically, nlrc3 activates the Notch pathway and the downstream gene of Notch hey1. Furthermore, NF-kB signaling acts upstream of nlrc3 to enhance its transcriptional activity. Finally, we find that Nlrc3 signaling is conserved in the regulation of murine embryonic hematopoiesis. Taken together, our findings uncover an indispensable role of Nlrc3 signaling in hematopoietic stem and progenitor cell emergence and provide insights into inflammation-related hematopoietic ontogeny and the in vitro expansion of hematopoietic stem and progenitor cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping