PUBLICATION
G-CSF induced hematopoietic stem cell mobilization from the embryonic hematopoietic niche does not require neutrophils and macrophages
- Authors
- Kim, J.W., Fedorov, E.A., Zon, L.I.
- ID
- ZDB-PUB-240103-20
- Date
- 2023
- Source
- Experimental hematology 131: 104147 (Journal)
- Registered Authors
- Zon, Leonard I.
- Keywords
- G-CSF, HSPC, macrophage, neutrophil, stem cell mobilization
- MeSH Terms
-
- Animals
- Granulocyte Colony-Stimulating Factor*/pharmacology
- Hematopoietic Stem Cell Mobilization/methods
- Hematopoietic Stem Cell Transplantation*
- Humans
- Macrophages/metabolism
- Neutrophils/metabolism
- Zebrafish/metabolism
- PubMed
- 38160994 Full text @ Exp. Hematol.
Citation
Kim, J.W., Fedorov, E.A., Zon, L.I. (2023) G-CSF induced hematopoietic stem cell mobilization from the embryonic hematopoietic niche does not require neutrophils and macrophages. Experimental hematology. 131:104147.
Abstract
Hematopoietic stem cell transplantation requires a collection of hematopoietic cells from patients or stem cell donors. Granulocyte colony-stimulating factor (G-CSF) is widely used in the clinic to mobilize hematopoietic stem and progenitor cells (HSPCs) from the adult bone marrow niche into circulation, allowing a collection of HSPCs from the blood. The mechanism by which G-CSF acts to mobilize HSPCs is unclear, with some studies showing a direct stimulation of stem cells and others suggesting that myeloid cells are required. In this study, we developed a heat-inducible G-CSF transgenic zebrafish line to study HSPC mobilization in vivo. Live imaging of HSPCs after G-CSF induction revealed an increase in circulating HSPCs, demonstrating a successful HSPC mobilization. These mobilized HSPCs went on to prematurely colonize the kidney marrow, the adult zebrafish hematopoietic niche. We eliminated neutrophils or macrophages using a nitroreductase-based cell ablation system and found that G-CSF still mobilizes HSPCs from the niche. Our findings indicate that neutrophils and macrophages are not required for G-CSF-induced HSPC mobilization from the embryonic hematopoietic niche.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping