PUBLICATION

In toto imaging of glial JNK signaling during larval zebrafish spinal cord regeneration

Authors
Becker, C.J., Cigliola, V., Gillotay, P., Rich, A., De Simone, A., Han, Y., Di Talia, S., Poss, K.D.
ID
ZDB-PUB-231124-12
Date
2023
Source
Development (Cambridge, England)   150(24): (Journal)
Registered Authors
Becker, Clay, Cigliola, Valentina, Gillotay, Pierre, Han, Yanchao, Poss, Kenneth D., Rich, Ashley
Keywords
JNK Signaling, Kinase translocation reporter, Regeneration, Spinal cord, Zebrafish
MeSH Terms
  • JNK Mitogen-Activated Protein Kinases
  • Neuroglia/physiology
  • Larva
  • Spinal Cord Regeneration*
  • Animals
  • Spinal Cord
  • Mammals
  • Zebrafish/physiology
  • Nerve Regeneration/physiology
  • Spinal Cord Injuries*
(all 10)
PubMed
37997694 Full text @ Development
Abstract
Identification of signaling events that contribute to innate spinal cord regeneration in zebrafish can inform new targets for modulating injury responses of the mammalian central nervous system. Using a chemical screen, we identify JNK signaling as a necessary regulator of glial cell cycling and tissue bridging during spinal cord regeneration in larval zebrafish. With a kinase translocation reporter, we visualize and quantify JNK signaling dynamics at single-cell resolution in glial cell populations in developing larvae and during injury-induced regeneration. Glial JNK signaling is patterned in time and space during development and regeneration, decreasing globally as the tissue matures and increasing in the rostral cord stump upon transection injury. Thus, dynamic and regional regulation of JNK signaling help to direct glial cell behaviors during innate spinal cord regeneration.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    pd390TgTransgenic Insertion
      pd391TgTransgenic Insertion
        pd392TgTransgenic Insertion
        w2
          		Point Mutation
          1 - 5 of 5
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          pcnaCRISPR4-pcnaCRISPR
          1 - 1 of 1
          Show
          Fish
          No data available
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          mCherryEFGmCherry
          VenusEFGVenus
          1 - 3 of 3
          Show
          Mapping
          No data available
          Your Input Welcome
          We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
          Citation
          Becker, C.J., Cigliola, V., Gillotay, P., Rich, A., De Simone, A., Han, Y., Di Talia, S., Poss, K.D. (2023) In toto imaging of glial JNK signaling during larval zebrafish spinal cord regeneration. Development (Cambridge, England). 150(24):.