PUBLICATION
Temporal and cellular analysis of granuloma development in mycobacterial infected adult zebrafish
- Authors
- Luo, G., Zeng, D., Liu, J., Li, D., Takiff, H.E., Song, S., Gao, Q., Yan, B.
- ID
- ZDB-PUB-231122-3
- Date
- 2023
- Source
- Journal of Leukocyte Biology 115(3): 525-535 (Journal)
- Registered Authors
- Keywords
- Tuberculosis, granuloma, macrophage, mycobacterium, pathology
- MeSH Terms
-
- Animals
- Granuloma/microbiology
- Granuloma/pathology
- Humans
- Mycobacterium*
- Mycobacterium Infections, Nontuberculous*
- Mycobacterium tuberculosis*
- Tuberculosis*
- Zebrafish/microbiology
- PubMed
- 37982587 Full text @ J. Leukoc. Biol.
Citation
Luo, G., Zeng, D., Liu, J., Li, D., Takiff, H.E., Song, S., Gao, Q., Yan, B. (2023) Temporal and cellular analysis of granuloma development in mycobacterial infected adult zebrafish. Journal of Leukocyte Biology. 115(3):525-535.
Abstract
Because granulomas are a hallmark of tuberculosis (TB) pathogenesis, the study of the dynamic changes in their cellular composition and morphological character can facilitate our understanding of TB pathogenicity. Adult zebrafish infected with Mycobacterium marinum (M. m) form granulomas that are similar to the granulomas in human TB patients and therefore have been used to study host-mycobacterium interactions. Most studies of zebrafish granulomas, however, have focused on necrotic granulomas, while a systematic description of the different stages of granuloma formation in the zebrafish model is lacking. Here, we characterized the stages of granulomas in M. m infected zebrafish, including early immune cell infiltration, non-necrotizing granulomas and necrotizing granulomas, using corresponding samples from pulmonary TB patients as references. We combined hematoxylin and eosin (H&E) staining and in situ hybridization (ISH) to identify the different immune cell types and follow their spatial distribution in the different stages of granuloma development. The macrophages in zebrafish granulomas were shown to belong to distinct subtypes: epithelioid macrophages, foamy macrophages, and multinucleated giant cells. By defining the developmental stages of zebrafish granulomas and the spatial distribution of the different immune cells they contain, this work provides a reference for future studies of mycobacterial granulomas and their immune microenvironments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping