PUBLICATION
Aspartate metabolic flux promotes nitric oxide to eliminate both antibiotic-sensitive and -resistant Edwardsiella tarda in zebrafish
- Authors
- Xiang, J., Li, M.Y., Li, H.
- ID
- ZDB-PUB-231027-64
- Date
- 2023
- Source
- Frontiers in immunology 14: 12772811277281 (Journal)
- Registered Authors
- Keywords
- Edwardsiella tarda, antibiotic-free approach, aspartate, nitric oxide, reprogramming metabolome, sodium nitroprusside, zebrafish
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents/pharmacology
- Aspartic Acid/pharmacology
- Bacteria
- Edwardsiella tarda
- Enterobacteriaceae Infections*
- Fish Diseases*
- Nitric Oxide
- Tetracyclines
- Zebrafish
- PubMed
- 37885884 Full text @ Front Immunol
Citation
Xiang, J., Li, M.Y., Li, H. (2023) Aspartate metabolic flux promotes nitric oxide to eliminate both antibiotic-sensitive and -resistant Edwardsiella tarda in zebrafish. Frontiers in immunology. 14:12772811277281.
Abstract
Introduction Metabolic reprogramming potentiates host protection against antibiotic-sensitive or -resistant bacteria. However, it remains unclear whether a single reprogramming metabolite is effective enough to combat both antibiotic-sensitive and -resistant bacteria. This knowledge is key for implementing an antibiotic-free approach.
Methods The reprogramming metabolome approach was adopted to characterize the metabolic state of zebrafish infected with tetracycline-sensitive and -resistant Edwardsiella tarda and to identify overlapping depressed metabolite in dying zebrafish as a reprogramming metabolite.
Results Aspartate was identify overlapping depressed metabolite in dying zebrafish as a reprogramming metabolite. Exogenous aspartate protects zebrafish against infection caused by tetracycline-sensitive and -resistant E. tarda. Mechanistically, exogenous aspartate promotes nitric oxide (NO) biosynthesis. NO is a well-documented factor of promoting innate immunity against bacteria, but whether it can play a role in eliminating both tetracycline-sensitive and -resistant E. tarda is unknown. Thus, in this study, aspartate was replaced with sodium nitroprusside to provide NO, which led to similar aspartate-induced protection against tetracycline-sensitive and -resistant E. tarda.
Discussion These findings support the conclusion that aspartate plays an important protective role through NO against both types of E. tarda. Importantly, we found that tetracycline-sensitive and -resistant E. tarda are sensitive to NO. Therefore, aspartate is an effective reprogramming metabolite that allows implementation of an antibiotic-free approach against bacterial pathogens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping