PUBLICATION
Protocol for controlling visual experience during zebrafish development and modulation of motor behavior
- Authors
- Hageter, J., Starkey, J., Barr, A., Huff, J.R., Horstick, E.J.
- ID
- ZDB-PUB-231015-68
- Date
- 2023
- Source
- STAR protocols 4: 102636102636 (Journal)
- Registered Authors
- Horstick, Eric
- Keywords
- Developmental Biology, Genetics, Model Organisms, Neuroscience
- MeSH Terms
-
- Animals
- Larva
- Sepharose
- Software*
- Zebrafish*
- PubMed
- 37837624 Full text @ STAR Protoc
Citation
Hageter, J., Starkey, J., Barr, A., Huff, J.R., Horstick, E.J. (2023) Protocol for controlling visual experience during zebrafish development and modulation of motor behavior. STAR protocols. 4:102636102636.
Abstract
Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity. For complete details on the use and execution of this protocol, please refer to Hageter et al. (2023).1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping