PUBLICATION
Generation and application of a novel transgenic zebrafish line Tg(GAcyp1a:eGFP/Luc) as an in vivo assay to sensitive and specific monitoring of DLCs in the environment
- Authors
- Xie, S., Yang, B., Li, S., Ge, L., Li, M., Chen, Q., Qing, X., Zou, J.
- ID
- ZDB-PUB-231002-69
- Date
- 2023
- Source
- Ecotoxicology and environmental safety 264: 115471 (Journal)
- Registered Authors
- Keywords
- DLCs, Environmental level, Mosquitofish, Transgenic zebrafish, cyp1a promoter
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Dibenzofurans/metabolism
- Dioxins*/metabolism
- Polychlorinated Dibenzodioxins*/metabolism
- Polychlorinated Dibenzodioxins*/toxicity
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 37729807 Full text @ Ecotoxicol. Environ. Saf.
Citation
Xie, S., Yang, B., Li, S., Ge, L., Li, M., Chen, Q., Qing, X., Zou, J. (2023) Generation and application of a novel transgenic zebrafish line Tg(GAcyp1a:eGFP/Luc) as an in vivo assay to sensitive and specific monitoring of DLCs in the environment. Ecotoxicology and environmental safety. 264:115471.
Abstract
CYP1A is the most commonly used biomarker and transgenic fish which carrying a cyp1a promoter to drive a reporter gene can be used as reliable way to monitor dioxin/dioxin-like compounds (DLCs) in the environment. Here, we cloned the cyp1a promoter of Gambusia affinis and this promoter showed stronger transcriptional activity than that of zebrafish. Then, a Tg(GAcyp1a:eGFP/Luc) transgenic zebrafish line was first constructed with the G. affinis cyp1a promoter driving eGFP expression using meganuclease I-SceI mediated transgenesis technology. The Tg(GAcyp1a:eGFP/Luc) larvae at 72 h post-fertilization (hpf) were tested by exposing to TCDD for 72 h, and induced GFP was mainly expressed in the liver with low background. The Tg(GAcyp1a:eGFP/Luc) zebrafish showed high sensitivity (limit of detection of 0.322 ng/L TCDD and 0.7 TEQ-ng/L PCDD/Fs) and specificity (insensitive to responses to PAHs and PCBs). In addition, the transgenic line showed a low detection concentration of the DLCs contaminated environmental samples (as low as 1.8 TEQ-ng/L), and the eGFP fluorescence intensity and the chemical-TEQ values were closely correlated. In conclusion, a sensitively and specifically transgenic zebrafish line was established to convenient and effective to detect DLCs in the environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping