PUBLICATION

Loss of the adaptor protein Sh3bgrl initiates ovarian fibrosis in zebrafish

Authors
Jin, Z., Wang, D., Lv, H., Wu, B., Li, Z., Guo, X., Wang, H., Yang, S.
ID
ZDB-PUB-230913-52
Date
2023
Source
FEBS letters   597(21): 2643-2655 (Journal)
Registered Authors
Yang, Shulan
Keywords
ECM, POI, ovarian fibrosis, sh3bgrl, zebrafish
MeSH Terms
  • Adaptor Proteins, Signal Transducing*/genetics
  • Animals
  • Female
  • Fibrosis
  • Ovary*
  • Zebrafish*/genetics
(all 6)
PubMed
37698355 Full text @ FEBS Lett.
Abstract
Ovarian fibrosis is a reproduction obstacle leading to female infertility in vertebrates, but the cause underlying the cellular events is unclear. Here, we found that the small adaptor protein SH3-domain-binding glutamate-rich protein like (Sh3bgrl) plays an important role in female reproduction in zebrafish. Two sh3bgrl mutant alleles that result in sh3bgrl depletion contributes to female spawning inability. Comparative transcriptome analysis revealed that sh3bgrl knockout mechanistically causes the upregulation of genes associated with extracellular matrix (ECM) and fiber generation in the zebrafish ovary. Consequently, extra ECM or fibers accumulate and are deposited in the ovary, resulting in eventual spawning inability. Our findings thus provide insights into understanding the underlying mechanism of infertility by ovarian fibrosis and provide a novel and valuable model to study female reproduction abnormality.
Genes / Markers
Marker Marker Type Name
col1a1bGENEcollagen, type I, alpha 1b
col1a2GENEcollagen, type I, alpha 2
col28a1aGENEcollagen, type XXVIII, alpha 1a
fgbGENEfibrinogen beta chain
sh3bgrlGENESH3 domain binding glutamate-rich protein like
sparcGENEsecreted protein, acidic, cysteine-rich (osteonectin)
1 - 6 of 6
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Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
syu503
    Insertion
    syu504
      Indel
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      sh3bgrlCRISPR1-sh3bgrlCRISPR
      1 - 1 of 1
      Show
      Fish
      No data available
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab4-acta2polyclonal
        IgGRabbit
        1 - 1 of 1
        Show
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available