PUBLICATION
Parkinson's disease model in zebrafish using intraperitoneal MPTP injection
- Authors
- Omar, N.A., Kumar, J., Teoh, S.L.
- ID
- ZDB-PUB-230911-61
- Date
- 2023
- Source
- Frontiers in neuroscience 17: 12360491236049 (Journal)
- Registered Authors
- Keywords
- Danio rerio, dopaminergic neuron, neurodegenerative disease, neurotoxin, tyrosine hydroxylase
- MeSH Terms
- none
- PubMed
- 37694115 Full text @ Front. Neurosci.
Citation
Omar, N.A., Kumar, J., Teoh, S.L. (2023) Parkinson's disease model in zebrafish using intraperitoneal MPTP injection. Frontiers in neuroscience. 17:12360491236049.
Abstract
Introduction Parkinson's disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic features of PD. This study aimed to assess the protocol for inducing PD following MPTP injection in adult zebrafish.
Methods Fish were injected with 100 μg/g of MPTP intraperitoneally once or twice and then assessed on days 1 to 30 post-injection.
Results Between one-time and two-time injections, there was no significant difference in most locomotor parameters, expressions of tyrosine hydroxylase-2 (th2) and dopamine transporter (dat) genes, and dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- and two-time injections on the day 1 assessment.
Discussion Over a 30-day period, the parameters showed significant differences in swimming speed, total distance traveled, tyrosine hydroxylase-1 (th1) and dat gene expressions, caspase-3 and glutathione protein levels, and TH+ cell counts. Days 3 and 5 showed the most changes compared to the control. In conclusion, a one-time injection of MPTP with delayed assessment on days 3 to 5 is a good PD model for animal studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping