PUBLICATION
Vertebral pattern and morphology is determined during embryonic segmentation
- Authors
- Serra, K.M., Vyzas, C., Shehreen, S., Chipendo, I., Clifford, K.M., Youngstrom, D.W., Devoto, S.H.
- ID
- ZDB-PUB-230910-63
- Date
- 2023
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 253(2): 204-214 (Journal)
- Registered Authors
- Devoto, Stephen Henri
- Keywords
- Tbx6, centra, scoliosis, somites, spine, vertebra
- MeSH Terms
-
- Animals
- Embryonic Development
- Mesoderm
- Somites
- Spine*/diagnostic imaging
- T-Box Domain Proteins/genetics
- Zebrafish*
- Zebrafish Proteins
- PubMed
- 37688793 Full text @ Dev. Dyn.
Citation
Serra, K.M., Vyzas, C., Shehreen, S., Chipendo, I., Clifford, K.M., Youngstrom, D.W., Devoto, S.H. (2023) Vertebral pattern and morphology is determined during embryonic segmentation. Developmental Dynamics : an official publication of the American Association of Anatomists. 253(2):204-214.
Abstract
Background The segmented nature of the adult vertebral column is based on segmentation of the paraxial mesoderm during early embryogenesis. Disruptions to embryonic segmentation, whether caused by genetic lesions or environmental stress, result in adult vertebral pathologies. However, the mechanisms linking embryonic segmentation and the details of adult vertebral morphology are poorly understood.
Results We induced border defects using two approaches in zebrafish: heat stress and misregulation of embryonic segmentation genes tbx6, mesp-ba, and ripply1. We assayed vertebral length, regularity, and polarity using microscopic and radiological imaging. In population studies, we find a correlation between specific embryonic border defects and specific vertebral defects, and within individual fish, we trace specific adult vertebral defects to specific embryonic border defects.
Conclusions Our data reveal that transient disruptions of embryonic segment border formation led to significant vertebral anomalies that persist through adulthood. The spacing of embryonic borders controls the length of the vertebra. The positions of embryonic borders control the positions of ribs and arches. Embryonic borders underlie fusions and divisions between adjacent spines and ribs. These data suggest that segment borders have a dominant role in vertebral development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping