PUBLICATION
Antioxidant, Pro-Survival and Pro-Regenerative Effects of Conditioned Medium from Wharton's Jelly Mesenchymal Stem Cells on Developing Zebrafish Embryos
- Authors
- Reina, C., Cardella, C., Lo Pinto, M., Pucci, G., Acuto, S., Maggio, A., Cavalieri, V.
- ID
- ZDB-PUB-230910-58
- Date
- 2023
- Source
- International Journal of Molecular Sciences 24(17): (Journal)
- Registered Authors
- Keywords
- Wharton’s jelly mesenchymal stem cells, antioxidant, bcl2, catalase, conditioned medium, histone post-translational modifications, ldha, nrf2, regeneration, zebrafish
- MeSH Terms
-
- Animals
- Antioxidants*/pharmacology
- Culture Media, Conditioned/pharmacology
- Mammals
- Reproducibility of Results
- Wharton Jelly*
- Zebrafish
- PubMed
- 37685998 Full text @ Int. J. Mol. Sci.
Citation
Reina, C., Cardella, C., Lo Pinto, M., Pucci, G., Acuto, S., Maggio, A., Cavalieri, V. (2023) Antioxidant, Pro-Survival and Pro-Regenerative Effects of Conditioned Medium from Wharton's Jelly Mesenchymal Stem Cells on Developing Zebrafish Embryos. International Journal of Molecular Sciences. 24(17):.
Abstract
Conditioned media harvested from stem cell culturing have the potential to be innovative therapeutic tools against various diseases, due to their high content of growth, trophic and protective factors. The evaluation in vivo of the effects and biosafety of these products is essential, and zebrafish provides an ideal platform for high-throughput toxicological analysis, concurrently allowing the minimization of the use of mammalian models without losing reliability. In this study, we assessed the biological effects elicited by the exposure of zebrafish embryos to a conditioned medium derived from Wharton's jelly mesenchymal stem cells. By a multiparametric investigation combining molecular, embryological, behavioural and in vivo imaging techniques, we found that exposure to a conditioned medium at a non-toxic/non-lethal dosage triggers antioxidant, anti-apoptotic and pro-regenerative effects, by upregulation of a set of genes involved in antioxidant defence (nrf2, brg1, sirt1, sirt6, foxO3a, sod2 and cat), glycolysis (ldha) and cell survival (bcl2l1, mcl1a and bim), coupled to downregulation of pro-apoptotic markers (baxa, caspase-3a and caspase-8). To our knowledge, this is the first study comprehensively addressing the effects of a conditioned medium on a whole organism from a developmental, molecular and behavioural perspective, and we are fairly confident that it will pave the way for future therapeutic application.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping