PUBLICATION
Microbial oil, alone or paired with β-glucans, can control hypercholesterolemia in a zebrafish model
- Authors
- Gora, A.H., Rehman, S., Dias, J., Fernandes, J.M.O., Olsvik, P.A., Sørensen, M., Kiron, V.
- ID
- ZDB-PUB-230902-53
- Date
- 2023
- Source
- Biochimica et biophysica acta. Molecular and cell biology of lipids 1868(11): 159383 (Journal)
- Registered Authors
- Fernandes, Jorge
- Keywords
- Dyslipidemia, Euglena, Functional diets, Microalgae glucans, Phaeodactylum, Schizochytrium, Zebrafish
- MeSH Terms
- none
- PubMed
- 37657755 Full text @ BBA Molecular and Cell Biology of Lipids
Citation
Gora, A.H., Rehman, S., Dias, J., Fernandes, J.M.O., Olsvik, P.A., Sørensen, M., Kiron, V. (2023) Microbial oil, alone or paired with β-glucans, can control hypercholesterolemia in a zebrafish model. Biochimica et biophysica acta. Molecular and cell biology of lipids. 1868(11):159383.
Abstract
Dyslipidemia is often associated with unhealthy dietary habits, and many mammalian studies have explored the mode of action of certain bioactive compounds such as β-glucans and n-3 PUFAs to understand their potential to normalize the lipid metabolism. There are only a few investigations of their combined effect on hypercholesterolemia by adopting omics approaches. Zebrafish (Danio rerio) was used as a model organism to reveal the efficacy of Schizochytrium oil and β-glucans (from Euglena gracilis and Phaeodactylum tricornutum). Four diets were fed to the fish: a control high-cholesterol diet or a Schizochytrium oil diet or one of the two diets containing the oil and β-glucan. The plasma HDL, expression of hepatic genes linked to, among others, ferric ion binding and plasma phosphatidylcholines were higher and plasma cholesterol esters and triacylglycerols were lower in the microbial oil-fed fish compared to the fish fed high cholesterol diet. While the fish fed a mix of microbial oil and Euglena-β-glucan had lower plasma triacylglycerols and expression of hepatic genes linked to PPAR signaling pathway and enriched biosynthesis of plasma unsaturated fatty acids, the fish fed microbial oil-Phaeodactylum-β-glucan combination had lower abundance of triacylglycerols rich in saturated and mono-unsaturated fatty acids and cholesterol esters in the plasma.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping