PUBLICATION
Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging
- Authors
- Zilliox, M., Tillement, V., Mangeat, T., Polès, S., Blader, P., Batut, J.
- ID
- ZDB-PUB-230826-50
- Date
- 2023
- Source
- STAR protocols 4: 102538102538 (Journal)
- Registered Authors
- Batut, Julie, Blader, Patrick, Polès, Sophie
- Keywords
- Cell Biology, Developmental Biology, Gene Expression, Microscopy, Model Organisms
- MeSH Terms
-
- Animals
- Light*
- Phenotype
- Zebrafish*/genetics
- PubMed
- 37624700 Full text @ STAR Protoc
Citation
Zilliox, M., Tillement, V., Mangeat, T., Polès, S., Blader, P., Batut, J. (2023) Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging. STAR protocols. 4:102538102538.
Abstract
Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development1 using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample preparation, live imaging, data collection, and analysis. This protocol can be applied to virtually any genetically modified experimental organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping