PUBLICATION
Furan based synthetic chalcone derivative functions against gut inflammation and oxidative stress demonstrated in in-vivo zebrafish model
- Authors
- Nayak, S.P.R.R., Dhivya, L.S., R, R., Almutairi, B.O., Arokiyaraj, S., Kathiravan, M.K., Arockiaraj, J.
- ID
- ZDB-PUB-230814-43
- Date
- 2023
- Source
- European Journal of Pharmacology 957: 175994 (Journal)
- Registered Authors
- Keywords
- Antioxidant, Chalcone, Gut inflammation, Inflammatory bowel disease, Oxidative stress
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/adverse effects
- Antioxidants/therapeutic use
- Chalcone*/pharmacology
- Chalcones*/pharmacology
- Colitis*/chemically induced
- Colitis*/drug therapy
- Colitis*/pathology
- Dextran Sulfate/adverse effects
- Inflammation/drug therapy
- Inflammatory Bowel Diseases*
- Oxidative Stress
- Zebrafish/metabolism
- PubMed
- 37574161 Full text @ Eur. J. Pharmacol.
Citation
Nayak, S.P.R.R., Dhivya, L.S., R, R., Almutairi, B.O., Arokiyaraj, S., Kathiravan, M.K., Arockiaraj, J. (2023) Furan based synthetic chalcone derivative functions against gut inflammation and oxidative stress demonstrated in in-vivo zebrafish model. European Journal of Pharmacology. 957:175994.
Abstract
Inflammatory Bowel Disease (IBD) is a group of persistent intestinal illnesses resulting from bowel inflammation unrelated to infection. The prevalence of IBD is rising in industrialized countries, increasing healthcare costs. Whether naturally occurring or synthetic, chalcones possess a broad range of biological properties, including anti-inflammatory, anti-microbial, and antioxidant effects. This investigation focuses on DKO7 (E)-3-(4-(dimethylamino)phenyl)-1-(5-methylfuran-2-yl)prop-2-en-1-one, a synthesized chalcone with potential anti-inflammatory effects in a zebrafish model of intestinal inflammation induced by Dextran sodium sulfate (DSS). The in vitro study displayed dose-dependent anti-inflammatory as well as antioxidant properties of DKO7. Additionally, DKO7 protected zebrafish larvae against lipid peroxidation, reactive oxygen stress (ROS), and DSS-induced inflammation. Moreover, DKO7 reduced the expression of pro-inflammatory genes, including TNF-α, IL-1β, IL-6, and iNOS. Further, it reduced the levels of nitric oxide (NO) and lactate dehydrogenase (LDH) in the intestinal tissues of adult zebrafish and increased the levels of antioxidant enzymes such as Catalase (CAT) and superoxide dismutase (SOD). The protective effect of DKO7 against chemically (or DSS) induced intestinal inflammation was further verified using histopathological techniques in intestinal tissues. The furan-based chalcone derivative, DKO7, displayed antioxidant and anti-inflammatory properties. Also, DKO7 successfully reverses the DSS-induced intestinal damage in zebrafish. Overall, this study indicates the ability of DKO7 to alleviate DSS-induced gut inflammation in an in-vivo zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping