PUBLICATION
ErbB2pY-1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification
- Authors
- Jin, M., Shi, R., Gao, D., Wang, B., Li, N., Li, X., Sik, A., Liu, K., Zhang, X.
- ID
- ZDB-PUB-230811-48
- Date
- 2023
- Source
- CNS neuroscience & therapeutics 30(2): e14407 (Journal)
- Registered Authors
- Keywords
- ErbB2, PD, SH-SY5Y, mouse, reverse phase protein array, zebrafish
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Dopamine/metabolism
- Dopaminergic Neurons/metabolism
- Lapatinib/metabolism
- Mice
- Mice, Inbred C57BL
- Parkinson Disease*/diagnosis
- Parkinson Disease*/metabolism
- Zebrafish
- PubMed
- 37564024 Full text @ CNS Neurosci Ther
Citation
Jin, M., Shi, R., Gao, D., Wang, B., Li, N., Li, X., Sik, A., Liu, K., Zhang, X. (2023) ErbB2pY-1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification. CNS neuroscience & therapeutics. 30(2):e14407.
Abstract
Aims This study aims to reveal a promising biomarker for Parkinson's disease (PD) based on research with reverse phase protein array (RPPA) technology for the first time and in vivo verification, which gains time for early intervention in PD, thus increasing the effectiveness of treatment and reducing disease morbidity.
Methods and results We employed RPPA technology which can assess both total and post-translationally modified proteins to identify biomarker candidates of PD in a cellular PD model. As a result, the phosphorylation (pY-1248) of the epidermal growth factor receptor (EGFR) ErbB2 is a promising biomarker candidate for PD. In addition, lapatinib, an ErbB2 tyrosine kinase inhibitor, was used to verify this PD biomarker candidate in vivo. We found that lapatinib-attenuated dopaminergic neuron loss and PD-like behavior in the zebrafish PD model. Accordingly, the expression of ErbB2pY-1248 significantly increased in the MPTP-induced mouse PD model. Our results suggest that ErbB2pY-1248 is a predictive biomarker for PD.
Conclusions In this study, we found that ErbB2pY-1248 is a predictive biomarker of PD by using RPPA technology and in vivo verification. It offers a new perspective on PD diagnosing and treatment, which will be essential in identifying individuals at risk of PD. In addition, this study provides new ideas for digging into biomarkers of other neurodegenerative diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping