PUBLICATION

A genetically encoded sensor for visualizing leukotriene B4 gradients in vivo

Authors

Tamás, S.X., Roux, B.T., Vámosi, B., Dehne, F.G., Török, A., Fazekas, L., Enyedi, B.

ID

ZDB-PUB-230802-45

Date

2023

Source

Nature communications 14: 46104610 (Journal)

Registered Authors

Enyedi, Balázs

Keywords

none

MeSH Terms

Animals
Humans
Leukotriene B4*/metabolism
Mice
Neutrophils
Receptors, Leukotriene B4/genetics
Receptors, Leukotriene B4/metabolism
Signal Transduction
Zebrafish*/genetics
Zebrafish*/metabolism

PubMed

37528073 Full text @ Nat. Commun.

Abstract

Leukotriene B₄ (LTB₄) is a potent lipid chemoattractant driving inflammatory responses during host defense, allergy, autoimmune and metabolic diseases. Gradients of LTB₄ orchestrate leukocyte recruitment and swarming to sites of tissue damage and infection. How LTB₄ gradients form and spread in live tissues to regulate these processes remains largely elusive due to the lack of suitable tools for monitoring LTB₄ levels in vivo. Here, we develop GEM-LTB₄, a genetically encoded green fluorescent LTB₄ biosensor based on the human G-protein-coupled receptor BLT1. GEM-LTB₄ shows high sensitivity, specificity and a robust fluorescence increase in response to LTB₄ without affecting downstream signaling pathways. We use GEM-LTB₄ to measure ex vivo LTB₄ production of murine neutrophils. Transgenic expression of GEM-LTB₄ in zebrafish allows the real-time visualization of both exogenously applied and endogenously produced LTB₄ gradients. GEM-LTB₄ thus serves as a broadly applicable tool for analyzing LTB₄ dynamics in various experimental systems and model organisms.

Genes / Markers

Figures

Expression

Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Tamás et al., 2023 ZDB-PUB-230802-45 PMID:37528073

A genetically encoded sensor for visualizing leukotriene B4 gradients in vivo

Tamás et al., 2023

ZDB-PUB-230802-45
PMID:37528073