PUBLICATION
Mechanical force regulates Sox9 expression at the developing enthesis
- Authors
- Subramanian, A., Kanzaki, L.F., Schilling, T.F.
- ID
- ZDB-PUB-230727-43
- Date
- 2023
- Source
- Development (Cambridge, England) 150(16): (Journal)
- Registered Authors
- Schilling, Tom
- Keywords
- Enthesis, Mechanotransduction, Tendon, Tenocyte differentiation
- MeSH Terms
-
- Animals
- Bone and Bones
- Ligaments
- Signal Transduction
- Tendons*/metabolism
- Zebrafish*/genetics
- PubMed
- 37497608 Full text @ Development
Citation
Subramanian, A., Kanzaki, L.F., Schilling, T.F. (2023) Mechanical force regulates Sox9 expression at the developing enthesis. Development (Cambridge, England). 150(16):.
Abstract
Entheses transmit force from tendons and ligaments to the skeleton. Regional organization of enthesis extracellular matrix (ECM) generates differences in stiffness required for force transmission. Two key transcription factors co-expressed in entheseal tenocytes, Scleraxis (Scx) and Sox9, directly control production of enthesis ECM components. Formation of embryonic craniofacial entheses in zebrafish coincides with onset of jaw movements, possibly in response to the force of muscle contraction. We show dynamic changes in scxa and sox9a mRNA levels in subsets of entheseal tenocytes that correlate with their roles in force transmission. We also show that transcription of a direct target of Scxa, Col1a, in enthesis ECM is regulated by the ratio of scxa to sox9a expression. Eliminating muscle contraction by paralyzing embryos during early stages of musculoskeletal differentiation alters relative levels of scxa and sox9a in entheses, primarily due to increased sox9a expression. Force-dependent TGF-beta (TGFb) signaling is required to maintain this balance of scxa and sox9a expression. Thus, force from muscle contraction helps establish a balance of transcription factor expression that controls specialized ECM organization at the tendon entheses and its ability to transmit force.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping