PUBLICATION
Role of short-wave-sensitive 1 (sws1) in cone development and first feeding in larval zebrafish
- Authors
- Lu, K., Liang, X.F., Tang, S.L., Wu, J., Zhang, L., Wang, Y., Chai, F.
- ID
- ZDB-PUB-230727-41
- Date
- 2023
- Source
- Fish physiology and biochemistry 49(5): 801-813 (Journal)
- Registered Authors
- Keywords
- Cone opsin, Food intake, Zebrafish, sws1
- MeSH Terms
-
- Animals
- Cone Opsins*/genetics
- Cone Opsins*/metabolism
- Opsins/genetics
- Opsins/metabolism
- Photoreceptor Cells, Vertebrate/metabolism
- Phylogeny
- Retinal Cone Photoreceptor Cells
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 37495865 Full text @ Fish Physiol. Biochem.
Citation
Lu, K., Liang, X.F., Tang, S.L., Wu, J., Zhang, L., Wang, Y., Chai, F. (2023) Role of short-wave-sensitive 1 (sws1) in cone development and first feeding in larval zebrafish. Fish physiology and biochemistry. 49(5):801-813.
Abstract
Color vision is mediated by the expression of different major visual pigment proteins (opsins) on retinal photoreceptors. Vertebrates have four classes of cone opsins that are most sensitive to different wavelengths of light: short wavelength sensitive 1 (SWS1), short wavelength sensitive 2 (SWS2), medium wavelength sensitive (RH2), and long wavelength sensitive (LWS). UV wavelengths play important roles in foraging and communication. However, direct evidence provide links between sws1 and first feeding is lacking. Here, CRISPR/Cas9 technology was performed to generate mutant zebrafish lines with sws1 deletion. sws1 mutant zebrafish larvae exhibited decreased sws1, rh2-2, and lws1 expression, and increased rod gene (rho and gnat1) expression. Furthermore, the sws1-deficient larvae exhibited significantly reduced food intake, and the orexigenic genes npy and agrp signaling were upregulated at 6 days postfertilization (dpf). The transcription expression of sws1 and rh2-3 genes decreased in sws1-/- adults compared to wild type. Surprisingly, the results of feeding at the adult stage were not the same with larvae. sws1 deficiency did not affect food intake and appetite gene expression at adult stages. These results reveal a role for sws1 in normal cone development and first feeding in larval zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping