PUBLICATION
Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1
- Authors
- Westerich, K.J., Tarbashevich, K., Schick, J., Gupta, A., Zhu, M., Hull, K., Romo, D., Zeuschner, D., Goudarzi, M., Gross-Thebing, T., Raz, E.
- ID
- ZDB-PUB-230719-39
- Date
- 2023
- Source
- Developmental Cell 58(17): 1578-1592.e5 (Journal)
- Registered Authors
- Goudarzi, Mohammad, Gross-Thebing, Theresa, Gupta, Antra, Raz, Erez, Schick, Jan, Tarbashevich, Katsiyarina
- Keywords
- Dead end, RNA translation, condensate, germ cells, germ granules, nanos, phase separation, zebrafish
- MeSH Terms
-
- Animals
- Gene Expression Regulation
- Germ Cells/metabolism
- Proteins/metabolism
- RNA*/genetics
- RNA*/metabolism
- Zebrafish*/metabolism
- PubMed
- 37463577 Full text @ Dev. Cell
Citation
Westerich, K.J., Tarbashevich, K., Schick, J., Gupta, A., Zhu, M., Hull, K., Romo, D., Zeuschner, D., Goudarzi, M., Gross-Thebing, T., Raz, E. (2023) Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1. Developmental Cell. 58(17):1578-1592.e5.
Abstract
Germ granules, condensates of phase-separated RNA and protein, are organelles that are essential for germline development in different organisms. The patterning of the granules and their relevance for germ cell fate are not fully understood. Combining three-dimensional in vivo structural and functional analyses, we study the dynamic spatial organization of molecules within zebrafish germ granules. We find that the localization of RNA molecules to the periphery of the granules, where ribosomes are localized, depends on translational activity at this location. In addition, we find that the vertebrate-specific Dead end (Dnd1) protein is essential for nanos3 RNA localization at the condensates' periphery. Accordingly, in the absence of Dnd1, or when translation is inhibited, nanos3 RNA translocates into the granule interior, away from the ribosomes, a process that is correlated with the loss of germ cell fate. These findings highlight the relevance of sub-granule compartmentalization for post-transcriptional control and its importance for preserving germ cell totipotency.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping