PUBLICATION
Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset
- Authors
- Mansur, A., Joseph, R., Kim, E., Jean-Beltran, P.M., Udeshi, N.D., Pearce, C., Jiang, H., Iwase, R., Milev, M.P., Almousa, H.A., McNamara, E., Widrick, J., Perez, C., Ravenscroft, G., Sacher, M., Cole, P.A., Carr, S.A., Gupta, V.A.
- ID
- ZDB-PUB-230712-42
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Gupta, Vandana A
- Keywords
- cell biology, developmental biology, zebrafish
- MeSH Terms
-
- Animals
- Disease Progression
- Endoplasmic Reticulum/metabolism
- Humans
- Muscle Development
- Muscle Proteins*/genetics
- Muscle Proteins*/metabolism
- Muscle, Skeletal/metabolism
- Proteome/metabolism
- Sarcomeres/metabolism
- Ubiquitin/metabolism
- Ubiquitination
- Zebrafish*/metabolism
- PubMed
- 37432316 Full text @ Elife
Citation
Mansur, A., Joseph, R., Kim, E., Jean-Beltran, P.M., Udeshi, N.D., Pearce, C., Jiang, H., Iwase, R., Milev, M.P., Almousa, H.A., McNamara, E., Widrick, J., Perez, C., Ravenscroft, G., Sacher, M., Cole, P.A., Carr, S.A., Gupta, V.A. (2023) Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset. eLIFE. 12:.
Abstract
Ubiquitin-proteasome system (UPS) dysfunction is associated with the pathology of a wide range of human diseases, including myopathies and muscular atrophy. However, the mechanistic understanding of specific components of the regulation of protein turnover during development and disease progression in skeletal muscle is unclear. Mutations in KLHL40, an E3 ubiquitin ligase cullin3 (CUL3) substrate-specific adapter protein, result in severe congenital nemaline myopathy, but the events that initiate the pathology and the mechanism through which it becomes pervasive remain poorly understood. To characterize the KLHL40-regulated ubiquitin-modified proteome during skeletal muscle development and disease onset, we used global, quantitative mass spectrometry-based ubiquitylome and global proteome analyses of klhl40a mutant zebrafish during disease progression. Global proteomics during skeletal muscle development revealed extensive remodeling of functional modules linked with sarcomere formation, energy, biosynthetic metabolic processes, and vesicle trafficking. Combined analysis of klh40 mutant muscle proteome and ubiquitylome identified thin filament proteins, metabolic enzymes, and ER-Golgi vesicle trafficking pathway proteins regulated by ubiquitylation during muscle development. Our studies identified a role for KLHL40 as a regulator of ER-Golgi anterograde trafficking through ubiquitin-mediated protein degradation of secretion-associated Ras-related GTPase1a (Sar1a). In KLHL40 deficient muscle, defects in ER exit site vesicle formation and downstream transport of extracellular cargo proteins result in structural and functional abnormalities. Our work reveals that the muscle proteome is dynamically fine-tuned by ubiquitylation to regulate skeletal muscle development and uncovers new disease mechanisms for therapeutic development in patients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping