PUBLICATION
Deficiency in hereditary hemorrhagic telangiectasia-associated Endoglin elicits hypoxia-driven heart failure in zebrafish
- Authors
- Lelièvre, E., Bureau, C., Bordat, Y., Frétaud, M., Langevin, C., Jopling, C., Kissa, K.
- ID
- ZDB-PUB-230602-39
- Date
- 2023
- Source
- Disease models & mechanisms 16(5): (Journal)
- Registered Authors
- Jopling, Chris, Kissa-Marin, Karima, Langevin, Christelle, Lelievre, Etienne
- Keywords
- Cardiomegaly, Endoglin, Endothelial cells, HHT, Heart failure, Hypoxia
- MeSH Terms
-
- Endothelial Cells/metabolism
- Endoglin/genetics
- Endoglin/metabolism
- Animals
- Zebrafish
- Telangiectasia, Hereditary Hemorrhagic*/complications
- Telangiectasia, Hereditary Hemorrhagic*/genetics
- Heart Failure*/metabolism
- Activin Receptors, Type II/genetics
- PubMed
- 37264878 Full text @ Dis. Model. Mech.
Citation
Lelièvre, E., Bureau, C., Bordat, Y., Frétaud, M., Langevin, C., Jopling, C., Kissa, K. (2023) Deficiency in hereditary hemorrhagic telangiectasia-associated Endoglin elicits hypoxia-driven heart failure in zebrafish. Disease models & mechanisms. 16(5):.
Abstract
Hereditary hemorrhagic telangiectasia (HHT) is a rare genetic disease caused by mutations affecting components of bone morphogenetic protein (BMP)/transforming growth factor-β (TGF-β) signaling in endothelial cells. This disorder is characterized by arteriovenous malformations that are prone to rupture, and the ensuing hemorrhages are responsible for iron-deficiency anemia. Along with activin receptor-like kinase (ALK1), mutations in endoglin are associated with the vast majority of HHT cases. In this study, we characterized the zebrafish endoglin locus and demonstrated that it produces two phylogenetically conserved protein isoforms. Functional analysis of a CRISPR/Cas9 zebrafish endoglin mutant revealed that Endoglin deficiency is lethal during the course from juvenile stage to adulthood. Endoglin-deficient zebrafish develop cardiomegaly, resulting in heart failure and hypochromic anemia, which both stem from chronic hypoxia. endoglin mutant zebrafish display structural alterations of the developing gills and underlying vascular network that coincide with hypoxia. Finally, phenylhydrazine treatment demonstrated that lowering hematocrit/blood viscosity alleviates heart failure and enhances the survival of Endoglin-deficient fish. Overall, our data link Endoglin deficiency to heart failure and establish zebrafish as a valuable HHT model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping