PUBLICATION
Preventive effect and mechanism of Tibetan tea extract on thrombosis in arachidonic acid-induced zebrafish determined via RNA-seq transcriptome profiles
- Authors
- Wang, N., Lan, C., Lu, H., Li, L., Liao, D., Xu, K., Sun, H., Tang, Y., Wang, Y., Mei, J., Wei, M., Wu, T., Zhu, H.
- ID
- ZDB-PUB-230520-29
- Date
- 2023
- Source
- PLoS One 18: e0285216e0285216 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Arachidonic Acid/metabolism
- Arachidonic Acid/pharmacology
- RNA-Seq
- Tea/metabolism
- Thrombosis*/drug therapy
- Thrombosis*/prevention & control
- Tibet
- Transcriptome*
- Zebrafish/metabolism
- PubMed
- 37205684 Full text @ PLoS One
Citation
Wang, N., Lan, C., Lu, H., Li, L., Liao, D., Xu, K., Sun, H., Tang, Y., Wang, Y., Mei, J., Wei, M., Wu, T., Zhu, H. (2023) Preventive effect and mechanism of Tibetan tea extract on thrombosis in arachidonic acid-induced zebrafish determined via RNA-seq transcriptome profiles. PLoS One. 18:e0285216e0285216.
Abstract
Thrombosis is a key pathological event in cardiovascular diseases and is also the most important targeting process for their clinical management. In this study, arachidonic acid (AA) was used to induce thrombus formation in zebrafish larvae. Blood flow, red blood cell (RBCs) aggregation and cellular oxidative stress were measured to evaluate the antithrombotic effect of Tibetan tea (TT). Meanwhile, the potential molecular mechanism was further explored by transcriptome sequencing (RNA-seq). The results indicated that TT could significantly restore heart RBCs intensity of thrombotic zebrafish, whilst decreasing RBCs accumulation in the caudal vein. The transcriptome analysis revealed that the preventive effect of TT on thrombosis could be mostly attributed to changes in lipid metabolism related signaling pathways, such as fatty acid metabolism, glycerollipid metabolism, ECM-receptor interaction and steroid biosynthesis signaling pathway. This study demonstrated that Tibetan tea could alleviate thrombosis by reducing oxidative stress levels and regulating lipid metabolism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping