PUBLICATION
Type I Diabetes in Zebrafish Reduces Sperm Quality and Increases Insulin and Glucose Transporter Transcripts
- Authors
- Diogo, P., Martins, G., Simão, M., Marreiros, A., Eufrásio, A.C., Cabrita, E., Gavaia, P.J.
- ID
- ZDB-PUB-230429-54
- Date
- 2023
- Source
- International Journal of Molecular Sciences 24(8): (Journal)
- Registered Authors
- Keywords
- DNA integrity, sperm motility, type I diabetes, zebrafish
- MeSH Terms
-
- Animals
- Cryopreservation
- DNA/metabolism
- Diabetes Mellitus, Type 1*/metabolism
- Glucose Transport Proteins, Facilitative/metabolism
- Humans
- Insulin/metabolism
- Insulin, Regular, Human
- Male
- Sperm Motility
- Spermatozoa/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 37108202 Full text @ Int. J. Mol. Sci.
Citation
Diogo, P., Martins, G., Simão, M., Marreiros, A., Eufrásio, A.C., Cabrita, E., Gavaia, P.J. (2023) Type I Diabetes in Zebrafish Reduces Sperm Quality and Increases Insulin and Glucose Transporter Transcripts. International Journal of Molecular Sciences. 24(8):.
Abstract
Type I diabetes is a prominent human pathology with increasing incidence in the population; however, its cause is still unknown. This disease promotes detrimental effects on reproduction, such as lower sperm motility and DNA integrity. Hence, the investigation of the underlying mechanisms of this metabolic disturbance in reproduction and its transgenerational consequences is of the utmost importance. The zebrafish is a useful model for this research considering its high homology with human genes as well as its fast generation and regeneration abilities. Therefore, we aimed to investigate sperm quality and genes relevant to diabetes in the spermatozoa of Tg(ins:nfsb-mCherry) zebrafish, a model for type I diabetes. Diabetic Tg(ins:nfsb-mCherry) males showed significantly higher expression of transcripts for insulin a (insa) and glucose transporter (slc2a2) compared to controls. Sperm obtained from the same treatment group showed significantly lower sperm motility, plasma membrane viability, and DNA integrity compared to that from the control group. Upon sperm cryopreservation, sperm freezability was reduced, which could be a consequence of poor initial sperm quality. Altogether, the data showed similar detrimental effects related to type I diabetes in zebrafish spermatozoa at the cellular and molecular levels. Therefore, our study validates the zebrafish model for type I diabetes research in germ cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping