PUBLICATION
Metabolic exploration of the developmental abnormalities and neurotoxicity of Esculentoside B, the main toxic factor in Phytolaccae radix
- Authors
- Ren, S., Zhang, Z., Song, Q., Ren, Z., Xiao, J., Li, L., Zhang, Q.
- ID
- ZDB-PUB-230421-60
- Date
- 2023
- Source
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 176: 113777 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Embryo, Nonmammalian
- Carboxylic Acids
- Larva
- Water Pollutants, Chemical*/metabolism
- Animals
- Zebrafish*
- PubMed
- 37080526 Full text @ Food Chem. Toxicol.
- CTD
- 37080526
Citation
Ren, S., Zhang, Z., Song, Q., Ren, Z., Xiao, J., Li, L., Zhang, Q. (2023) Metabolic exploration of the developmental abnormalities and neurotoxicity of Esculentoside B, the main toxic factor in Phytolaccae radix. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 176:113777.
Abstract
P: radix is a perennial herb, and its extracts have various biological properties that make it a potential candidate for the treatment of tumors, edema, and lymphatic stasis. However, the main factor contributing to its toxicity are not clear. Here, we used a zebrafish toxicological model to study the main toxicity factor of P. radix and explore the potential mechanisms involved. The results revealed that Esculentoside B was the major toxic factor of P. radix. Exposure of zebrafish larvae to Esculentoside B caused developmental abnormalities, neurotoxicity and altered locomotor behavior. The combination of AChE activity and the expression levels of genes relevant to CNS development demonstrated that Esculentoside B is neurotoxic to zebrafish larvae, impairs their CNS development, and that AChE may be a toxic target of Esculentoside B. Metabolomic analysis has revealed that Esculentoside B exposure can disrupt D-Amino acid metabolism, protein export, autophagy, and mTOR signaling pathways in zebrafish larvae. These findings provide insights into the molecular mechanisms underlying EsB-induced neurotoxicity in zebrafish, which can facilitate further research and development of P. radix for safe consumption.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping