PUBLICATION
CRISPR/Cas9-Mediated Genome Editing in Zebrafish
- Authors
- Daniel, J.G., Yu, X., Ferguson, A.C., Shavit, J.A.
- ID
- ZDB-PUB-230331-49
- Date
- 2023
- Source
- Methods in molecular biology (Clifton, N.J.) 2631: 371380371-380 (Chapter)
- Registered Authors
- Daniel, Jeffrey G., Ferguson, Allison, Shavit, Jordan, Yu, Xinge
- Keywords
- CRISPR/Cas9, Genome editing, Mutagenesis, Zebrafish
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems*/genetics
- Gene Editing*
- Genome
- Zebrafish/genetics
- PubMed
- 36995678 Full text @ Meth. Mol. Biol.
Citation
Daniel, J.G., Yu, X., Ferguson, A.C., Shavit, J.A. (2023) CRISPR/Cas9-Mediated Genome Editing in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2631:371380371-380.
Abstract
The CRISPR/Cas9 system is a powerful tool for genome editing in zebrafish. This workflow takes advantage of the genetic tractability of zebrafish and will allow users to edit genomic sites and produce mutant lines using selective breeding. Established lines may then be employed by researchers for downstream genetic and phenotypic analyses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping