PUBLICATION

Temporally and regionally distinct morphogenetic processes govern zebrafish caudal fin blood vessel network expansion

Authors
Leonard, E.V., Hasan, S.S., Siekmann, A.F.
ID
ZDB-PUB-230321-40
Date
2023
Source
Development (Cambridge, England)   150(7): (Journal)
Registered Authors
Siekmann, Arndt Friedrich
Keywords
Angiogenesis, Artery Vein Morphogenesis, Blood vessel, Caudal fin, Cxcr4, Notch, VEGF, Zebrafish
MeSH Terms
  • Neovascularization, Physiologic
  • Endothelial Cells*
  • Veins/metabolism
  • Vascular Endothelial Growth Factor A/metabolism
  • Animals
  • Zebrafish*/genetics
(all 6)
PubMed
36938965 Full text @ Development
Abstract
Blood vessels form elaborate networks depending on tissue-specific signalling pathways and anatomical structures to guide their growth. However, it is not clear which morphogenetic principles organize the stepwise assembly of the vasculature. We thus performed a longitudinal analysis of zebrafish caudal fin vascular assembly, revealing the existence of temporally and spatially distinct morphogenetic processes. Initially, vein-derived endothelial cells (ECs) generated arteries in a reiterative process requiring Vascular Endothelial Growth Factor (VEGF), Notch and cxcr4a signalling. Subsequently, veins produced veins in more proximal fin regions, transforming pre-existing artery-vein loops into a three-vessel pattern consisting of an artery and two veins. A distinct set of vascular plexuses formed at the base of the fin. They differed by virtue of diameter, flow magnitude and marker gene expression. At later stages, intussusceptive angiogenesis occurred from veins in distal fin regions. In proximal fin regions, we observed new vein sprouts crossing the inter-ray tissue through sprouting angiogenesis. Together, our results reveal a surprising diversity among the mechanisms generating the mature fin vasculature and suggest that these might be driven by separate local cues.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    b1212TgTransgenic Insertion
      ca8TgTransgenic Insertion
        cq27TgTransgenic Insertion
          hu5088
            		Point Mutation
            hu5333TgTransgenic Insertion
              mu104TgTransgenic Insertion
                mu128
                  		Small Deletion
                  mu240TgTransgenic Insertion
                    ncv24TgTransgenic Insertion
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Orthology
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CitrineEFGCitrine
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GAL4FFEFGGAL4FF
                      mCherryEFGmCherry
                      RFPEFGRFP
                      VenusEFGVenus
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                      Citation
                      Leonard, E.V., Hasan, S.S., Siekmann, A.F. (2023) Temporally and regionally distinct morphogenetic processes govern zebrafish caudal fin blood vessel network expansion. Development (Cambridge, England). 150(7).