PUBLICATION
11.4 T ultra-high static magnetic field has no effect on morphology but induces upregulation of TNF signaling pathway based on transcriptome analysis in zebrafish embryos
- Authors
- Liu, C., Lu, S., Liu, S., Dong, C., Chen, Y., Xiao, L., Zong, Y., Zhang, H., Liao, A.
- ID
- ZDB-PUB-230318-39
- Date
- 2023
- Source
- Ecotoxicology and environmental safety 255: 114754 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Left-Right asymmetry, RNA-seq, TNF signaling pathway, UHF
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian*
- Gene Expression Profiling
- Signal Transduction/genetics
- Tumor Necrosis Factors/metabolism
- Up-Regulation
- Zebrafish*/metabolism
- PubMed
- 36931084 Full text @ Ecotoxicol. Environ. Saf.
Citation
Liu, C., Lu, S., Liu, S., Dong, C., Chen, Y., Xiao, L., Zong, Y., Zhang, H., Liao, A. (2023) 11.4 T ultra-high static magnetic field has no effect on morphology but induces upregulation of TNF signaling pathway based on transcriptome analysis in zebrafish embryos. Ecotoxicology and environmental safety. 255:114754.
Abstract
As magnetic resonance imaging (MRI) scanners with ultra-high field (UHF) have optimal performance, scientists have been working to develop high-performance devices with strong magnetic fields to improve their diagnostic potential. However, whether an MRI scanner with UHF poses a risk to the safety of the organism require further evaluation. This study evaluated the effects of 11.4 Tesla (T) UHF on embryonic development using a zebrafish model. Multiple approaches, including morphological parameters, physiological behaviors, and analyses of the transcriptome at the molecular level, were determined during 5 days after laboratory-controlled exposure from 6 hour post fertilization (hpf) to 24 hpf. No significant effects were observed in embryo mortality, hatching rate, body length, Left-Right patterning, locomotor behavior, etc. RNA-sequencing analysis revealed up-regulated tumor necrosis factor (TNF) inflammatory factors and activated TNF signaling pathways in the 11.4 T exposure group. The results were further validated using qPCR. Our findings indicate that although UHF exposure under 11.4 T has no effect on the development of zebrafish embryos, it has specific effects on the immune response that require further investigation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping