PUBLICATION

Mob4 is required for neurodevelopment in zebrafish

Authors: Florindo, C., Mimoso, J.M., Palma, S.L., Gonçalves, C., Silvestre, D., Campinho, M., Tavares, Á.A.
ID: ZDB-PUB-230315-36
Date: 2023
Source: microPublication. Biology 2023: (Journal)
Registered Authors: Campinho, Marco António
Keywords: none
MeSH Terms: none
PubMed: 36915897 Full text @ MicroPubl Biol

Abstract

Mob4 is an essential evolutionary conserved protein shown to play roles in cell division and neural development. Mob4 is a core component of the macromolecular STRIPAK complex involved in various critical cellular processes, from cell division to signal transduction pathways. However, Mob4 remains relatively poorly understood. Although the consequences of eliminating Mob4 function in Drosophila are described, its function in vertebrate development remains largely unknown. Here we show that knockdown and knockout of Mob4 during zebrafish embryogenesis limits neuronal cell divisions but has little effect on apoptosis, thus arguing a role for mob4 in neurodevelopment.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
mob4	AB	standard conditions	20-25 somites	brain	ISH	Fig. 1
mob4	AB	standard conditions	Long-pec	brain	ISH	Fig. 1
mob4	AB	standard conditions	Prim-5	brain	ISH	Fig. 1
mob4	AB	standard conditions	Prim-25	brain	ISH	Fig. 1
mob4	AB	standard conditions	26+ somites	brain	ISH	Fig. 1
mob4	AB	standard conditions	Long-pec	caudal vein plexus	ISH	Fig. 1
mob4	AB	standard conditions	Long-pec	dorsal aorta	ISH	Fig. 1
mob4	AB	standard conditions	Long-pec	heart	ISH	Fig. 1
mob4	AB	standard conditions	Prim-25	heart tube	ISH	Fig. 1
mob4	AB	standard conditions	90%-epiboly	neuroectoderm	ISH	Fig. 1

1 - 10 of 16

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + CRISPR2-mob4	standard conditions	Prim-5	brain lacks all parts of type midbrain hindbrain boundary, abnormal	Fig. 1
WT + CRISPR2-mob4	standard conditions	Prim-5	eye decreased size, abnormal	Fig. 1
WT + CRISPR2-mob4	standard conditions	Prim-5	midbrain hindbrain boundary aplastic, abnormal	Fig. 1
WT + MO1-mob4	standard conditions	Prim-5	brain lacks all parts of type midbrain hindbrain boundary, abnormal	Fig. 1
WT + MO1-mob4	standard conditions	Prim-5	eye cell population proliferation decreased occurrence, abnormal	Fig. 1
WT + MO1-mob4	standard conditions	Prim-5	eye decreased size, abnormal	Fig. 1
WT + MO1-mob4	standard conditions	Prim-5	head decreased size, abnormal	Fig. 1
WT + MO1-mob4	standard conditions	Prim-5	hindbrain cell population proliferation decreased occurrence, abnormal	Fig. 1
WT + MO1-mob4	standard conditions	Prim-5	hindbrain decreased area, abnormal	Fig. 1
WT + MO1-mob4	standard conditions	Prim-5	midbrain hindbrain boundary aplastic, abnormal	Fig. 1

1 - 10 of 11

Show

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
mob4	CRISPR2-mob4	CRISPR
mob4	MO1-mob4	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
AB
WT + CRISPR2-mob4
WT + MO1-mob4
WT + MO1-mob4 + MO4-tp53

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab2-mob4	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Mapping

Florindo et al., 2023 ZDB-PUB-230315-36 PMID:36915897

Mob4 is required for neurodevelopment in zebrafish

Florindo et al., 2023

ZDB-PUB-230315-36
PMID:36915897