PUBLICATION
A single-cell resolution gene expression atlas of the larval zebrafish brain
- Authors
- Shainer, I., Kuehn, E., Laurell, E., Al Kassar, M., Mokayes, N., Sherman, S., Larsch, J., Kunst, M., Baier, H.
- ID
- ZDB-PUB-230223-70
- Date
- 2023
- Source
- Science advances 9: eade9909eade9909 (Journal)
- Registered Authors
- Al Kassar, Mariam, Baier, Herwig, Kunst, Michael, Larsch, Johannes, Laurell, Eva, Mokayes, Nouwar, Shainer, Inbal, Sherman, Shachar
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain*/physiology
- Gene Expression
- Larva
- Neurons/metabolism
- Zebrafish*/metabolism
- PubMed
- 36812331 Full text @ Sci Adv
Citation
Shainer, I., Kuehn, E., Laurell, E., Al Kassar, M., Mokayes, N., Sherman, S., Larsch, J., Kunst, M., Baier, H. (2023) A single-cell resolution gene expression atlas of the larval zebrafish brain. Science advances. 9:eade9909eade9909.
Abstract
The advent of multimodal brain atlases promises to accelerate progress in neuroscience by allowing in silico queries of neuron morphology, connectivity, and gene expression. We used multiplexed fluorescent in situ RNA hybridization chain reaction (HCR) technology to generate expression maps across the larval zebrafish brain for a growing set of marker genes. The data were registered to the Max Planck Zebrafish Brain (mapzebrain) atlas, thus allowing covisualization of gene expression, single-neuron tracings, and expertly curated anatomical segmentations. Using post hoc HCR labeling of the immediate early gene cfos, we mapped responses to prey stimuli and food ingestion across the brain of freely swimming larvae. This unbiased approach revealed, in addition to previously described visual and motor areas, a cluster of neurons in the secondary gustatory nucleus, which express the marker calb2a, as well as a specific neuropeptide Y receptor, and project to the hypothalamus. This discovery exemplifies the power of this new atlas resource for zebrafish neurobiology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping