PUBLICATION
Loss of Agrp1 in zebrafish: Effects on the growth and reproductive axis
- Authors
- Wei, Z., Bagivalu Lakshminarasimha, A., Cone, R., Michel, M.
- ID
- ZDB-PUB-230223-33
- Date
- 2023
- Source
- General and comparative endocrinology 336: 114243 (Journal)
- Registered Authors
- Michel, Max
- Keywords
- AgRP, FSH, GH, LH, zebrafish
- MeSH Terms
-
- Agouti-Related Protein/genetics
- Agouti-Related Protein/metabolism
- Animals
- Follicle Stimulating Hormone*/genetics
- Gonadotropins
- Growth Hormone/genetics
- Growth Hormone/metabolism
- Luteinizing Hormone
- Mammals/metabolism
- Zebrafish*/metabolism
- PubMed
- 36801393 Full text @ Gen. Comp. Endocrinol.
Citation
Wei, Z., Bagivalu Lakshminarasimha, A., Cone, R., Michel, M. (2023) Loss of Agrp1 in zebrafish: Effects on the growth and reproductive axis. General and comparative endocrinology. 336:114243.
Abstract
Loss of agouti related neuropeptide (AgRP) does not lead to overt phenotypes in mammals unless AgRP neurons are ablated. In contrast, in zebrafish it has been shown that Agrp1 loss of function (LOF) leads to reduced growth in Agrp1 morphant as well as Agrp1 mutant larvae. Further, it has been shown that multiple endocrine axes are dysregulated upon Agrp1 LOF in Agrp1 morphant larvae. Here we show that adult Agrp1 LOF zebrafish show normal growth and reproductive behavior in spite of a significant reduction in multiple related endocrine axes namely reduced expression in pituitary growth hormone (gh) follicle stimulating hormone (fshb) as well as luteinizing hormone (lhb). We looked for compensatory changes in candidate gene expression but found no changes in growth hormone and gonadotropin hormone receptors that would explain the lack of phenotype. We further looked at expression in the hepatic and muscular insulin-like growth factor (Igf) axis which appears to be normal. Fecundity as well as ovarian histology also appears largely normal while we do see an increase in mating efficiency specifically in fed but not fasted AgRP1 LOF animals. This data shows that zebrafish can grow and reproduce normally in spite of significant central hormone changes and suggests a peripheral compensatory mechanism additional to previously reported central compensatory mechanisms in other zebrafish neuropeptide LOF lines.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping