PUBLICATION
Otolin-1, an otolith- and otoconia-related protein, controls calcium carbonate bioinspired mineralization
- Authors
- Bielak, K., Benkowska-Biernacka, D., Ptak, M., Stolarski, J., Kalka, M., O?yhar, A., Dobryszycki, P.
- ID
- ZDB-PUB-230216-36
- Date
- 2023
- Source
- Biochimica et biophysica acta. General subjects 1867(5): 130327 (Journal)
- Registered Authors
- Keywords
- Biocrystal, Biomineral, Biomineralization, Calcium carbonate, Otoconia, Otolin-1, Otoliths
- MeSH Terms
-
- Otolithic Membrane*/chemistry
- Otolithic Membrane*/metabolism
- Calcium Carbonate*/chemistry
- Extracellular Matrix Proteins/metabolism
- Humans
- Zebrafish/metabolism
- Animals
- PubMed
- 36791829 Full text @ BBA General Subjects
Citation
Bielak, K., Benkowska-Biernacka, D., Ptak, M., Stolarski, J., Kalka, M., O?yhar, A., Dobryszycki, P. (2023) Otolin-1, an otolith- and otoconia-related protein, controls calcium carbonate bioinspired mineralization. Biochimica et biophysica acta. General subjects. 1867(5):130327.
Abstract
Background Otoliths and otoconia are calcium carbonate biomineral structures that form in the inner ear of fish and humans, respectively. The formation of these structures is tightly linked to the formation of an organic matrix framework with otolin-1, a short collagen-like protein from the C1q family as one of its major constituents.
Methods In this study, we examined the activity of recombinant otolin-1 originating from Danio rerio and Homo sapiens on calcium carbonate bioinspired mineralization with slow-diffusion method and performed crystals characterization with scanning electron microscopy, two-photon excited fluorescence microscopy, confocal laser scanning microscopy and micro-Raman spectroscopy.
Results We show that both proteins are embedded in the core of CaCO3 crystals that form through the slow-diffusion mineralization method. Both of them influence the morphology but do not change the polymorphic mineral phase. D.rerio otolin-1 also closely adheres to the crystal surface.
General significance The results suggest, that otolin-1 is not a passive scaffold, but is directly involved in regulating the morphology of the resulting calcium carbonate biocrystals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping