PUBLICATION

Triclocarban triggers osteoarthritis via DNMT1-mediated epigenetic modification and suppression of COL2A in cartilage tissues

Authors
Zhang, Y., He, L., Yang, Y., Cao, J., Su, Z., Zhang, B., Guo, H., Wang, Z., Zhang, P., Xie, J., Li, J., Ye, J., Zha, Z., Yu, H., Hong, A., Chen, X.
ID
ZDB-PUB-230122-32
Date
2023
Source
Journal of hazardous materials   447: 130747130747 (Journal)
Registered Authors
Keywords
Cartilage, Collagen, DNA methylation, Osteoarthritis, Triclocarban
MeSH Terms
  • Animals
  • Cartilage, Articular*/metabolism
  • Cartilage, Articular*/pathology
  • Collagen Type II/genetics
  • Collagen Type II/metabolism
  • DNA (Cytosine-5-)-Methyltransferase 1/genetics
  • DNA (Cytosine-5-)-Methyltransferase 1/metabolism
  • Epigenesis, Genetic
  • Humans
  • Osteoarthritis*/chemically induced
  • Osteoarthritis*/genetics
  • Osteoarthritis*/metabolism
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
PubMed
36680903 Full text @ J. Hazard. Mater.
Abstract
Triclocarban (TCC) is a widely used environmental endocrine-disrupting chemical (EDC). Articular injury of EDCs has been reported; however, whether and how TCCs damage the joint have not yet been determined. Herein, we revealed that exposure to TCC caused osteoarthritis (OA) within the zebrafish anal fin. Mechanistically, TCC stimulates the expression of DNMT1 and initiates DNA hypermethylation of the type II collagen coding gene, which further suppresses the expression of type II collagen and other extracellular matrices. This further results in decreased cartilage tissue and narrowing of the intraarticular space, which is typical of the pathogenesis of OA. The regulation of OA occurrence by TCC is conserved between zebrafish cartilage tissue and human chondrocytes. Our findings clarified the hazard and potential mechanisms of TCC towards articular health and highlighted DNMT1 as a potential therapeutic target for OA caused by TCC.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping