PUBLICATION
Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of Lycium barbarum Polysaccharides
- Authors
- Zeng, W., Chen, L., Xiao, Z., Li, Y., Ma, J., Ding, J., Yang, J.
- ID
- ZDB-PUB-230122-26
- Date
- 2023
- Source
- Molecules 28(2): (Journal)
- Registered Authors
- Keywords
- Lycium barbarum polysaccharides, antioxidant activity, chemical characteristics, immunomodulatory activity, molecular weight
- MeSH Terms
-
- Animals
- Drugs, Chinese Herbal*/chemistry
- Glucose
- Lycium*/chemistry
- Molecular Weight
- Polysaccharides/chemistry
- Polysaccharides/pharmacology
- Zebrafish
- PubMed
- 36677759 Full text @ Molecules
Citation
Zeng, W., Chen, L., Xiao, Z., Li, Y., Ma, J., Ding, J., Yang, J. (2023) Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of Lycium barbarum Polysaccharides. Molecules. 28(2):.
Abstract
The molecular weight, the triple-helix conformation, the monosaccharide content, the manner of glycosidic linkages, and the polysaccharide conjugates of polysaccharides all affect bioactivity. The purpose of this study was to determine how different molecular weights affected the bioactivity of the Lycium barbarum polysaccharides (LBPs). By ethanol-graded precipitation and ultrafiltration membrane separation, one oligosaccharide (LBPs-1, 1.912 kDa) and two polysaccharides (LBPs-2, 7.481 kDa; LBPs-3, 46.239 kDa) were obtained from Lycium barbarum. While the major component of LBPs-1 and LBPs-2 was glucose, the main constituents of LBPs-3 were arabinose, galactose, and glucose. LBPs-2 and LBPs-3 exhibited triple-helix conformations, as evidenced by the Congo red experiment and AFM data. Sugar residues of LBPs-2 and LBPs-3 were elucidated by NMR spectra. The polysaccharides (LBPs-2 and LBPs-3) exhibited much higher antioxidant capacities than oligosaccharide (LBPs-1). LBPs-3 showed higher oxygen radical absorbance capacity (ORAC) and superoxide dismutase (SOD) activity than LBPs-2, but a lower capability for scavenging ABTS+ radicals. In zebrafish, LBPs-2 and LBPs-3 boosted the growth of T-lymphocytes and macrophages, enhanced the immunological response, and mitigated the immune damage generated by VTI. In addition to the molecular weight, the results indicated that the biological activities would be the consequence of various aspects, such as the monosaccharide composition ratio, the chemical composition, and the chemical reaction mechanism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping