PUBLICATION
An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish
- Authors
- Sy, S.K.H., Chan, D.C.W., Chan, R.C.H., Lyu, J., Li, Z., Wong, K.K.Y., Choi, C.H.J., Mok, V.C.T., Lai, H.M., Randlett, O., Hu, Y., Ko, H.
- ID
- ZDB-PUB-230115-6
- Date
- 2023
- Source
- Nature communications 14: 227227 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Brain*/physiology
- Zebrafish*/physiology
- Animals
- Larva
- Microscopy, Fluorescence/methods
- Cadaverine
- PubMed
- 36641479 Full text @ Nat. Commun.
Citation
Sy, S.K.H., Chan, D.C.W., Chan, R.C.H., Lyu, J., Li, Z., Wong, K.K.Y., Choi, C.H.J., Mok, V.C.T., Lai, H.M., Randlett, O., Hu, Y., Ko, H. (2023) An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish. Nature communications. 14:227227.
Abstract
Studying chemosensory processing desires precise chemical cue presentation, behavioral response monitoring, and large-scale neuronal activity recording. Here we present Fish-on-Chips, a set of optofluidic tools for highly-controlled chemical delivery while simultaneously imaging behavioral outputs and whole-brain neuronal activities at cellular resolution in larval zebrafish. These include a fluidics-based swimming arena and an integrated microfluidics-light sheet fluorescence microscopy (µfluidics-LSFM) system, both of which utilize laminar fluid flows to achieve spatiotemporally precise chemical cue presentation. To demonstrate the strengths of the platform, we used the navigation arena to reveal binasal input-dependent behavioral strategies that larval zebrafish adopt to evade cadaverine, a death-associated odor. The µfluidics-LSFM system enables sequential presentation of odor stimuli to individual or both nasal cavities separated by only ~100 µm. This allowed us to uncover brainwide neural representations of cadaverine sensing and binasal input summation in the vertebrate model. Fish-on-Chips is readily generalizable and will empower the investigation of neural coding in the chemical senses.
Errata / Notes
This article is corrected by ZDB-PUB-230507-44.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping