PUBLICATION
Lactobacillus delbrueckii reduces anxiety-like behavior in zebrafish through a gut microbiome - Brain crosstalk
- Authors
- Olorocisimo, J.P., Diaz, L.A., Co, D.E., Carag, H.M., Ibana, J.A., Velarde, M.C.
- ID
- ZDB-PUB-221225-4
- Date
- 2022
- Source
- Neuropharmacology 225: 109401 (Journal)
- Registered Authors
- Keywords
- Anxiolytic, Danio rerio, Gut-brain axis, Lactic acid bacteria, Psychobiotics
- MeSH Terms
-
- Gastrointestinal Microbiome*
- Brain/metabolism
- Zebrafish/genetics
- Zebrafish/microbiology
- Anxiety
- RNA, Ribosomal, 16S/genetics
- RNA, Ribosomal, 16S/metabolism
- Lactobacillus delbrueckii*/genetics
- Lactobacillus delbrueckii*/metabolism
- Animals
- PubMed
- 36565853 Full text @ Neuropharmacology
Citation
Olorocisimo, J.P., Diaz, L.A., Co, D.E., Carag, H.M., Ibana, J.A., Velarde, M.C. (2022) Lactobacillus delbrueckii reduces anxiety-like behavior in zebrafish through a gut microbiome - Brain crosstalk. Neuropharmacology. 225:109401.
Abstract
Certain bacteria possess the ability to reduce anxiety- and stress-related behaviors through the gut microbiome-brain axis. Such bacteria are called psychobiotics, and can be used to improve mood and cognition. However, only a few bacteria have been characterized as psychobiotics, and their exact mechanism of action remains unclear. Hence, in this study we analyzed three different species under the Lactobacillacea family, namely, Lactobacillus delbrueckii, Lacticaseibacillus casei, and Lacticaseibacillus paracasei for their potential psychobiotic activities. L. delbrueckii treatment reduced anxiety-like behavior and increased brain and gut glutamic acid decarboxylase (gad) gene expression in zebrafish. It also altered zebrafish gut microbial community as determined by PCR-DGGE and 16S rRNA-based metagenomics analysis. Overall, this paper showed that L. delbrueckii but not L. paracasei and L. casei, induced a consistent improvement in anxiety-like behavior in zebrafish, implicating its potential role as a psychobiotic to reduce anxiety.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping