PUBLICATION
Oligodendrocyte origin and development in the zebrafish visual system
- Authors
- Santos-Ledo, A., Pérez-Montes, C., DeOliveira-Mello, L., Arévalo, R., Velasco, A.
- ID
- ZDB-PUB-221209-11
- Date
- 2022
- Source
- The Journal of comparative neurology 531(4): 515-527 (Journal)
- Registered Authors
- Keywords
- Sox, development, myelination, oligodendrocytes, visual system, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Cell Differentiation/physiology
- Mammals
- Myelin Sheath/physiology
- Oligodendrocyte Transcription Factor 2/metabolism
- Oligodendroglia*/metabolism
- SOXE Transcription Factors/genetics
- SOXE Transcription Factors/metabolism
- Zebrafish*/metabolism
- PubMed
- 36477827 Full text @ J. Comp. Neurol.
Citation
Santos-Ledo, A., Pérez-Montes, C., DeOliveira-Mello, L., Arévalo, R., Velasco, A. (2022) Oligodendrocyte origin and development in the zebrafish visual system. The Journal of comparative neurology. 531(4):515-527.
Abstract
Oligodendrocytes are the myelinating cells in the central nervous system. In birds and mammals, the oligodendrocyte progenitor cells (OPCs) originate in the preoptic area (POA) of the hypothalamus. However, it remains unclear in other vertebrates such as fish. Thus, we have studied the early progression of OPCs during zebrafish visual morphogenesis from 2 days post fertilization (dpf) until 11 dpf using the olig2:EGFP transgenic line; and we have analyzed the differential expression of transcription factors involved in oligodendrocyte differentiation: Sox2 (using immunohistochemistry) and Sox10 (using the transgenic line sox10:tagRFP). The first OPCs (olig2:EGFP/Sox2) were found at 2 dpf in the POA. From 3 dpf onwards, these olig2:EGFP/Sox2 cells migrate to the optic chiasm, where they invade the optic nerve (ON), extending toward the retina. At 5 dpf, olig2:EGFP/Sox2 cells in the ON also colocalize with sox10:tagRFP. When olig2:EGFP cells differentiate and present more projections, they become positive only for sox10:tagRFP. olig2:EGFP/sox10: tagRFP cells ensheath the ON by 5 dpf when they also become positive for a myelin marker, based on the mbpa:tagRFPt transgenic line. We also found olig2:EGFP cells in other regions of the visual system. In the central retina at 2 dpf, they are positive for Sox2 but later become restricted to the proliferative germinal zone without this marker. In the ventricular areas of the optic tectum, olig2:EGFP cells present Sox2 but arborized ones sox10:tagRFP instead. Our data matches with other models, where OPCs are specified in the POA and migrate to the ON through the optic chiasm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping