PUBLICATION

Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development

Authors
Xiao, Y., Chen, J., Yang, S., Sun, H., Xie, L., Li, J., Jing, N., Zhu, X.
ID
ZDB-PUB-221209-10
Date
2022
Source
The EMBO journal   42(3): e111364 (Journal)
Registered Authors
Keywords
deadenylation, embryogenesis, maternal mRNA, maternal-to-zygotic transition, phase separation
Datasets
GEO:GSE179035
MeSH Terms
  • Animals
  • Mice
  • Pregnancy
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • RNA, Messenger, Stored*/genetics
  • RNA, Messenger, Stored*/metabolism
  • Embryonic Development/genetics
  • Blastula/metabolism
  • Blastocyst/metabolism
  • Female
  • Zebrafish*
  • Gene Expression Regulation, Developmental
(all 13)
PubMed
36477743 Full text @ EMBO J.
Abstract
Early embryonic development depends on proper utilization and clearance of maternal transcriptomes. How these processes are spatiotemporally regulated remains unclear. Here we show that nuclear RNA-binding protein Rbm14 and maternal mRNAs co-phase separate into cytoplasmic condensates to facilitate vertebrate blastula-to-gastrula development. In zebrafish, Rbm14 condensates were highly abundant in blastomeres and markedly reduced after prominent activation of zygotic transcription. They concentrated at spindle poles by associating with centrosomal γ-tubulin puncta and displayed mainly asymmetric divisions with a global symmetry across embryonic midline in 8- and 16-cell embryos. Their formation was dose-dependently stimulated by m6 A, but repressed by m5 C modification of the maternal mRNA. Furthermore, deadenylase Parn co-phase separated with these condensates, and this was required for deadenylation of the mRNAs in early blastomeres. Depletion of Rbm14 impaired embryonic cell differentiations and full activations of the zygotic genome in both zebrafish and mouse and resulted in developmental arrest at the blastula stage. Our results suggest that cytoplasmic Rbm14 condensate formation regulates early embryogenesis by facilitating deadenylation, protection, and mitotic allocation of m6 A-modified maternal mRNAs, and by releasing the poly(A)-less transcripts upon regulated disassembly to allow their re-polyadenylation and translation or clearance.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Xiao, Y., Chen, J., Yang, S., Sun, H., Xie, L., Li, J., Jing, N., Zhu, X. (2022) Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development. The EMBO journal. 42(3):e111364.